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产品描述：人B-淋巴细胞趋化因子1(BLC-1/CXCL13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL13含量。CXCL13是一个重要靶点。它是趋化因子，在免疫细胞迁移、淋巴器官发育构建等生理过程起关键作用。其研究机制聚焦于它与受体CXCR5的相互作用，二者结合参与多种疾病发展，如自身免疫病、癌症，是药物研发热点。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。适用于基础研究领域，如评估疾病模型中B细胞募集机制、探索感染或肿瘤微环境中趋化因子网络动态变化，以及相关生物标志物的体外定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ANGIE ELISA Kit; ANGIE2 ELISA Kit; B cell attracting chemokine 1 ELISA Kit; B cell-attracting chemokine 1 ELISA Kit; B lymphocyte chemoattractant ELISA Kit; B-cell chemoattractant ELISA Kit; B-cell-attracting chemokine 1 ELISA Kit; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1) ELISA Kit; BCA-1 ELISA Kit; BLC ELISA Kit; BLR1L ELISA Kit; C-X-C motif chemokine 13 ELISA Kit; Chemokine (C-X-C motif) ligand 13 ELISA Kit; Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant) ELISA Kit; Chemokine; CXC motif; ligand 13 ELISA Kit; CXC chemokine BLC ELISA Kit; CXCL13 ELISA Kit; CXL13_HUMAN ELISA Kit; SCYB13 ELISA Kit; Small inducible cytokine B subfamily (Cys-X-Cys motif); member 13 (B-cell chemoattractant) ELISA Kit; Small inducible cytokine B13 ELISA Kit; Small inducible cytokine subfamily B; member 13 ELISA Kit; Small-inducible cytokine B13 ELISA Kit
缩写：CXCL13
Uniprot No.：O43927
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BLC-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-99 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 83-104 | |||||
回收率：
| The recovery of human BLC-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-105 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 50002.8042.8562.8302.661 25002.1322.0472.0901.921 12501.3391.3461.3431.174 6250.8230.8390.8310.662 3120.4760.4840.4800.311 1560.3260.3420.3340.165 780.2470.2510.2490.080 00.1670.1700.169 | ||||||
货期：3-5 working days
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