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产品描述：人干扰素诱导蛋白10(IP-10/CXCL10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL10含量。CXCL10属于趋化因子，在炎症、免疫反应等生理病理过程中发挥重要作用。研究显示它可与CXCR3受体结合，调控免疫细胞趋化与活化，在肿瘤、自身免疫病等疾病中异常表达，成为潜在治疗靶点，围绕其开展的干预研究具有重要意义。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.8 pg/mL。适用于基础科研中探索炎症相关信号通路、评估疾病模型免疫状态、分析组织微环境细胞因子网络等场景，为免疫调控机制研究、药物干预效果评估及生物标志物筛选提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Interferon gamma induced factor MOB1; mouse; homolog of ELISA Kit; Interferon gamma induced protein 10 ELISA Kit; 10 kDa interferon gamma induced protein ELISA Kit; 10 kDa interferon gamma-induced protein ELISA Kit; C X C motif chemokine 10 ELISA Kit; C7 ELISA Kit; Chemokine (C X C motif) ligand 10 ELISA Kit; Chemokine CXC motif ligand 10 ELISA Kit; Crg 2 ELISA Kit; CRG2 ELISA Kit; CXCL10 ELISA Kit; CXCL10(1-73) ELISA Kit; CXL10_HUMAN ELISA Kit; Gamma IP10 ELISA Kit; Gamma-IP10 ELISA Kit; gIP 10 ELISA Kit; GIP10 ELISA Kit; IFI10 ELISA Kit; INP 10 ELISA Kit; INP10 ELISA Kit; Interferon activated gene 10 ELISA Kit; Interferon activated gene 10 ELISA Kit; Interferon gamma induced cell line ELISA Kit; Interferon inducible cytokine IP 10 ELISA Kit; Interferon inducible cytokine IP10 ELISA Kit; IP 10 ELISA Kit; IP-10 ELISA Kit; Mob 1 ELISA Kit; MOB1 ELISA Kit; Protein 10 from interferon (gamma) induced cell line ELISA Kit; SCYB10 ELISA Kit; Small inducible cytokine B10 ELISA Kit; Small inducible cytokine B10 precursor ELISA Kit; Small inducible cytokine subfamily B (Cys X Cys) member 10 ELISA Kit; Small inducible cytokine subfamily B CXC member 10 ELISA Kit; Small inducible cytokine subfamily B; member 10 ELISA Kit; Small-inducible cytokine B10 ELISA Kit
缩写：CXCL10
Uniprot No.：P02778
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of human IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 93 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.9232.9632.9432.832 10002.1512.1762.1642.053 5001.2551.2761.2661.155 2500.7060.6710.6890.578 1250.4340.3990.4170.306 62.50.2990.2850.2920.181 31.250.2480.2530.2510.140 00.1100.1110.111 | ||||||
货期：3-5 working days
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