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Human Fractalkine,FK ELISA Kit

  • 中文名: 人趋化因子(FK)ELISA Kit
货号: IPD-E04558h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人趋化因子(FK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CX3CL1含量。CX3CL1,也称为Fractalkine,是一种与炎症疾病相关的趋化因子,存在于两种形式:膜锚定的或脱落的糖蛋白。CX3CL1在炎症条件下在单核细胞中表达,并与多种疾病如类风湿性关节炎、动脉粥样硬化等的发生相关。其受体CX3CR1是一种7次跨膜受体,介导CX3CL1的粘附和迁移功能,并参与多种临床疾病的发病机制。试剂盒检测范围为0.312 ng/mL-20 ng/mL,灵敏度为0.078 ng/mL。该产品适用于科研领域中对免疫调控机制、炎症相关疾病动物模型评估、药物干预效果追踪等研究,尤其为体外实验或体内模型中FK动态变化的检测提供稳定工具。满足不同实验场景下对趋化因子活性研究的精准需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    A 152E5.2 ELISA Kit; AB030188 ELISA Kit; ABCD 3 ELISA Kit; ABCD3 ELISA Kit; AI848747 ELISA Kit; C-X3-C motif chemokine 1 ELISA Kit; C3Xkine ELISA Kit; Chemokine (C-X3-C motif) ligand 1 ELISA Kit; Chemokine C X3 C motif ligand 1 ELISA Kit; Chemokine CX3C Motif Ligand 1 ELISA Kit; CX3C membrane anchored chemokine ELISA Kit; CX3C membrane-anchored chemokine ELISA Kit; Cx3cl1 ELISA Kit; CXC 3 ELISA Kit; CXC3 ELISA Kit; CXC3C ELISA Kit; D8Bwg0439e ELISA Kit; FKN ELISA Kit; Fractalkine ELISA Kit; Neurotactin ELISA Kit; NTN ELISA Kit; NTT ELISA Kit; Processed fractalkine ELISA Kit; SCYD 1 ELISA Kit; SCYD1 ELISA Kit; Small inducible cytokine D1 ELISA Kit; Small inducible cytokine subfamily D (Cys X3 Cys) member 1 ELISA Kit; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) ELISA Kit; Small inducible cytokine subfamily D member 1 ELISA Kit; Small-inducible cytokine D1 ELISA Kit; X3CL1_HUMAN ELISA Kit
  • 缩写:
    CX3CL1
  • Uniprot No.:
    P78423
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.312 ng/mL-20 ng/mL
  • 灵敏度:
    0.078 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human FK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    88


    Range %

    82-99


    1:2

    Average %

    97


    Range %

    91-102


    1:4

    Average %

    90


    Range %

    84-100


    1:8

    Average %

    95


    Range %

    88-98


  • 回收率:

    The recovery of human FK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    85-98


    EDTA plasma (n=4)

    87

    81-95


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    2.420

    2.533

    2.477

    2.328


    10

    1.535

    1.567

    1.551

    1.403


    5

    0.870

    0.933

    0.901

    0.753


    2.5

    0.595

    0.603

    0.599

    0.450


    1.25

    0.316

    0.321

    0.319

    0.170


    0.625

    0.221

    0.235

    0.228

    0.080


    0.312

    0.193

    0.204

    0.199

    0.050


    0

    0.152

    0.145

    0.148




  • 货期:
    3-5 working days

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