Rat cartilage oligomeric matrix protein,COMP ELISA Kit

大包装询价

货号: IPD-E13833r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠软骨寡聚基质蛋白(COMP)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的COMP含量。软骨寡聚基质蛋白（COMP）是一种细胞外基质蛋白，主要存在于软骨中，对软骨细胞分化和基质形成起重要作用。研究发现，COMP在颞下颌关节滑膜软骨瘤病（TMJSC）中表达显著增高，可能与TGF-β3、TGF-β1、BMP-2等细胞因子协同作用，促进滑膜细胞软骨化生及软骨增殖，从而在TMJSC发病中起一定作用。试剂盒检测范围为78 pg/ml- 5000 pg/ml，灵敏度为19.5 pg/ml。本试剂盒可为骨关节疾病机制研究、软骨损伤修复模型评估、骨质疏松相关药物开发等科研领域提供定量分析工具，通过检测体液或组织中的COMP含量变化，助力研究者深入探索软骨代谢调控网络及病理生理学机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
CompCartilage oligomeric matrix protein ELISA kit; COMP ELISA kit
缩写：
COMP
Uniprot No.：
P35444
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/ml - 5000 pg/ml
灵敏度：
19.5 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cell Biology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat COMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-107
1:2	Average %	97
	Range %	91-105
1:4	Average %	98
	Range %	92-104
1:8	Average %	100
	Range %	95-105

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	2.639	2.699	2.669	2.501
2500	2.151	2.163	2.157	1.989
1250	1.505	1.542	1.524	1.356
625	1.062	1.098	1.080	0.912
312	0.637	0.689	0.663	0.495
156	0.392	0.399	0.396	0.228
78	0.332	0.347	0.340	0.172
0	0.168	0.167	0.168

A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat COMP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled COMP antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.