Human cartilage oligomeric protein,COMP ELISA Kit

中文名： 人软骨寡聚蛋白(COMP)ELISA Kit

货号: IPD-E09138h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人软骨寡聚蛋白(COMP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COMP含量。试剂盒检测范围为0.156 ng/ml-10 ng/ml，灵敏度为0.039 ng/ml。科研人员可将其应用于骨关节炎病理机制研究、软骨组织工程再生评估、运动医学中关节损伤修复监测等基础研究领域，为探索软骨相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
cartilage oligomeric matrix protein (pseudoachondroplasia; epiphyseal dysplasia 1; multiple) ELISA Kit; Cartilage oligomeric matrix protein ELISA Kit; Cartilage oligomeric matrix protein precursor ELISA Kit; COMP ELISA Kit; COMP_HUMAN ELISA Kit; EDM 1 ELISA Kit; EDM1 ELISA Kit; EPD 1 ELISA Kit; EPD1 ELISA Kit; Epiphyseal dysplasia 1 ELISA Kit; Epiphyseal dysplasia 1 multiple ELISA Kit; Epiphyseal dysplasia multiple 1 ELISA Kit; MED ELISA Kit; MGC13181 ELISA Kit; MGC149768 ELISA Kit; PSACH ELISA Kit; pseudoachondroplasia (epiphyseal dysplasia 1; multiple) ELISA Kit; Pseudoachondroplasia ELISA Kit; THBS 5 ELISA Kit; THBS5 ELISA Kit; Thrombospondin 5 ELISA Kit; Thrombospondin-5 ELISA Kit; Thrombospondin5 ELISA Kit; TSP5 ELISA Kit
缩写：
COMP
Uniprot No.：
P49747
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.156 ng/ml-10 ng/ml
灵敏度：
0.039 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cell Biology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)1.1811.2181.2501.1811.2041.241SD0.0350.0380.0450.0350.0400.051CV(%)4.7234.9805.7544.7235.2986.564

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human COMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:100

Average %

Range %

83-92

1:200

Average %

Range %

85-95

1:400

Average %

Range %

87-97

1:800

Average %

Range %

90-100

回收率：

The recovery of human COMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	93	88-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.












ng/ml	OD1	OD2	Average	Corrected
10	2.710	2.631	2.671	2.567
5	2.214	2.151	2.183	2.079
2.5	1.463	1.398	1.431	1.327
1.25	0.795	0.759	0.777	0.673
0.625	0.448	0.441	0.445	0.341
0.312	0.267	0.247	0.257	0.153
0.156	0.179	0.173	0.176	0.072
0	0.106	0.101	0.104