Mouse Complement fragment 5a,C5a ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08514m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠补体片断5a(C5a)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C5a含量。C5a是补体系统激活过程中产生的重要炎症介质。它在免疫调节、炎症反应等方面发挥关键作用。研究机制主要聚焦于其与受体结合后引发的细胞信号传导通路，如激活免疫细胞、促进趋化等，可作为炎症相关疾病治疗的潜在靶点。试剂盒检测范围为39 pg/mL-2500 pg/mL，灵敏度为9.75 pg/mL。适用于体外实验中对炎症模型、免疫调控机制或药物干预效果的评估，为研究补体系统相关疾病的分子机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
C5a
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
39 pg/mL-2500 pg/mL
灵敏度：
9.75 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:20	Average %	85
	Range %	81-90
1:40	Average %	98
	Range %	91-105
1:80	Average %	89
	Range %	84-94
1:160	Average %	90
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2500	2.844	2.734	2.789	2.662
1250	2.331	2.197	2.264	2.137
625	1.488	1.528	1.508	1.381
312.5	0.810	0.829	0.819	0.693
156.25	0.546	0.521	0.533	0.407
78	0.357	0.363	0.360	0.234
39	0.221	0.214	0.218	0.091
0	0.128	0.125	0.127