Human Complement fragment 5a,C5a ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08512h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人补体片断5a(C5a)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C5a含量。C5a是补体系统激活后产生的重要活性片段。其背景在于补体系统是天然免疫重要组成部分。研究机制上，C5a可与相应受体结合，在炎症反应、免疫调节等过程中发挥关键作用，参与多种疾病发生发展，是药物研发潜在重要靶点。试剂盒检测范围为39 pg/ml-2500 pg/ml，灵敏度为9.75 pg/ml。科研人员可通过定量评估C5a在感染模型、自身免疫疾病研究或药物干预实验中的动态变化，为炎症机制研究、免疫调节因子筛选及病理模型验证提供数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
N/A
缩写：
C5a
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
39 pg/ml-2500 pg/ml
灵敏度：
9.75 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	102
	Range %	92-108
1:200	Average %	94
	Range %	89-99
1:400	Average %	97
	Range %	91-104
1:800	Average %	94
	Range %	89-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2500	2.991	3.107	3.049	2.920
1250	2.476	2.457	2.467	2.338
625	1.423	1.363	1.393	1.264
312	0.657	0.643	0.650	0.521
156	0.327	0.329	0.328	0.199
78	0.208	0.202	0.205	0.076
39	0.171	0.163	0.167	0.038
0	0.131	0.127	0.129