首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human Complement 4,C4 ELISA Kit

  • 中文名： 人补体蛋白4(C4)ELISA Kit
货号: IPD-E08705h
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：人补体蛋白4(C4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C4含量。试剂盒检测范围为0.41 ng/mL-300 ng/mL，灵敏度为0.41 ng/mL。适用于基础医学研究中C4蛋白的动态监测，例如感染性疾病模型的补体激活机制分析、自身免疫性疾病动物模型的病理机制探索，或药物干预下补体通路的功能研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Acidic C4 ELISA Kit; Acidic complement C4 ELISA Kit; Basic C4 ELISA Kit; Basic complement C4 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit; C4; Chido form ELISA Kit; C4; Rodgers from ELISA Kit; C4A anaphylatoxin ELISA Kit; C4A ELISA Kit; C4A2 ELISA Kit; C4A3 ELISA Kit; C4A4 ELISA Kit; C4A6 ELISA Kit; C4AD ELISA Kit; C4B ELISA Kit; C4B_2 ELISA Kit; C4B1 ELISA Kit; C4B12 ELISA Kit; C4B2 ELISA Kit; C4B3 ELISA Kit; C4BD ELISA Kit; C4F ELISA Kit; C4S ELISA Kit; CH ELISA Kit; Chido form of C4 ELISA Kit; CO4 ELISA Kit; CO4A_HUMAN ELISA Kit; Complement C4 A ELISA Kit; Complement C4 B ELISA Kit; Complement C4 gamma chain ELISA Kit; complement C4-A ELISA Kit; complement C4-B ELISA Kit; complement C4-B-like ELISA Kit; complement C4B1a ELISA Kit; Complement component 4A (Rodgers blood group) ELISA Kit; Complement component 4A ELISA Kit; complement component 4B (Chido blood group) ELISA Kit; complement component 4B (Chido blood group); copy 2 ELISA Kit; Complement component 4B (Childo blood group) ELISA Kit; Complement component 4B ELISA Kit; Complement component 4F ELISA Kit; Complement component 4S ELISA Kit; CPAMD2 ELISA Kit; CPAMD3 ELISA Kit; RG ELISA Kit; Rodgers form of C4 ELISA Kit

  • 缩写：C4

  • Uniprot No.：P0C0L4

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, tissue homogenates

  • 检测范围：0.41 ng/mL-300 ng/mL

  • 灵敏度：0.41 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Others

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:1000Average %96
    Range %93-99
    1:2000Average %100
    Range %97-103
    1:4000Average %89
    Range %86-92
    1:8000Average %100
    Range %97-104

  • 回收率：

    The recovery of human C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)8683-90
    EDTA plasma (n=4)9591-99














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected 3002.8732.8972.8852.786 1001.9161.9681.9421.843 33.31.2811.2991.2901.191 11.10.8780.8980.8880.789 3.70.4950.4720.4840.385 1.230.1950.2120.2040.105 0.410.1870.1920.1900.091 00.1010.0970.099　 





  • 本试剂盒所含材料：



    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human C4 antibody.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial HRP-conjugated C4 antibody (100 x concentrate) (120 μl/bottle) ---Bind to the C4 in the samples or standards and react with the substrate to make the solution chromogenic.

    • One HRP-conjugate Diluent(20 ml/bottle) ---Dilute the HRP-conjugated C4 antibody

    • Two vials Sample Diluent (20 ml/bottle) ---Dilute the sample solution.

    • One vial Wash Buffer (25x concentrate) (20ml/bottle) ---Wash away unbound or free substances.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：3-5 working days

      客户数据及评论

      折叠内容

      大包装询价

      ×