Mouse Osteocalcin/Bone gla protein,OT/BGP ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E06917m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠骨钙素/骨谷氨酸蛋白(OT/BGP)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的BGLAP含量。BGLAP在骨代谢中扮演重要角色。它是骨组织特异性蛋白，参与骨矿化过程。研究聚焦于其基因表达调控、与骨细胞相互作用机制，旨在揭示其在骨发育、稳态维持及相关疾病如骨质疏松发病机理中的作用，为骨病防治提供新靶点。试剂盒检测范围为78 pg/ml- 5000 pg/ml，灵敏度为19.5 pg/ml。适用于科研领域中对骨代谢相关机制的探索，例如骨质疏松模型研究、骨再生药物效果评估、骨骼发育动态监测等场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Bglap ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
缩写：
BGLAP
Uniprot No.：
P86546
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/ml - 5000 pg/ml
灵敏度：
19.5 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	94
	Range %	85-102
1:2	Average %	95
	Range %	91-100
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	1.768	1.634	1.701	1.521
2500	1.245	1.247	1.246	1.066
1250	0.791	0.828	0.809	0.629
625	0.490	0.489	0.490	0.309
312	0.381	0.361	0.371	0.191
156	0.324	0.313	0.318	0.138
78	0.236	0.244	0.240	0.060
0	0.185	0.175	0.180