首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit

  • 中文名： 人骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA Kit
货号: IPD-E05128h
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：
    人骨钙素/骨谷氨酸蛋白(OT/BGP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BGLAP含量。BGLAP（骨钙素）是一种由成骨细胞分泌的激素，参与骨骼重塑和能量代谢。其羧化形式负向调节骨形成，同时促进骨吸收和矿化，对磷灰石和钙有强亲和力。研究显示，BGLAP在骨骼发育、成骨细胞分化和生物矿化组织中发挥重要作用。试剂盒检测范围为31.25 pg/ml-2000 pg/ml，灵敏度为7.8 pg/ml。适用于骨代谢相关基础研究，如骨质疏松症动物模型评估、骨骼发育机制探索、骨组织工程材料生物活性分析等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    BGLAP ELISA Kit; BGP ELISA Kit; Bone gamma carboxyglutamate (gla) protein ELISA Kit; Bone gamma carboxyglutamate gla protein osteocalcin ELISA Kit; Bone gamma carboxyglutamate protein ELISA Kit; Bone Gla protein ELISA Kit; Gamma carboxyglutamic acid containing protein ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit; OC ELISA Kit; OCN ELISA Kit; OSTCN_HUMAN ELISA Kit; Osteocalcin ELISA Kit; OTTHUMP00000016586 ELISA Kit; PMF1 ELISA Kit
  • 缩写：
    BGLAP
  • Uniprot No.：
    P02818
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/ml-2000 pg/ml
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    98


    Range %

    95-106


    1:2

    Average %

    99


    Range %

    93-105


    1:4

    Average %

    94


    Range %

    86-99


    1:8

    Average %

    97


    Range %

    91-105


  • 回收率：

    The recovery of human OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    99

    96-103


    EDTA plasma (n=4)

    97

    94-101


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    2000

    2.552

    2.634

    2.593

    2.483


    1000

    1.764

    1.667

    1.716

    1.605


    500

    1.242

    1.228

    1.235

    1.125


    250

    0.747

    0.729

    0.738

    0.628


    125

    0.476

    0.451

    0.463

    0.353


    62.5

    0.324

    0.333

    0.329

    0.218


    31.25

    0.194

    0.184

    0.189

    0.079


    0

    0.111

    0.109

    0.110



  • 本试剂盒所含材料：



    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human BGLAP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled BGLAP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：


      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate

      • 货期：
        3-5 working days

      客户数据及评论

      折叠内容

      大包装询价

      ×