首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人载脂蛋白B100(Apo-B100)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、唾液、尿液样本中的APOB含量。载脂蛋白B（APOB）是乳糜微粒、低密度脂蛋白和极低密度脂蛋白的关键成分，由人类APOB基因编码。研究显示，APOB与心血管疾病、血脂异常等密切相关，其研究机制涉及脂质代谢、炎症反应和动脉粥样硬化过程。试剂盒检测范围为78 ng/mL-5000 ng/mL，灵敏度为19.5 ng/mL。科研领域可用于探索脂蛋白代谢机制、心血管疾病模型建立、药物筛选或生物标志物研究等方向，例如通过检测不同生理或病理条件下（如高脂饮食、基因修饰模型）样本中的Apo - B100表达水平，为脂代谢调控及疾病机制解析提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apo B 100 ELISA Kit; Apo B ELISA Kit; Apo B-100 ELISA Kit; Apo B-48 ELISA Kit; Apo B100 ELISA Kit; Apo B48 ELISA Kit; ApoB 100 ELISA Kit; ApoB 48 ELISA Kit; APOB ELISA Kit; APOB_HUMAN ELISA Kit; Apolipoprotein B (including Ag(x) antigen) ELISA Kit; Apolipoprotein B 100 ELISA Kit; Apolipoprotein B 48 ELISA Kit; Apolipoprotein B ELISA Kit; Apolipoprotein B-48 ELISA Kit; Apolipoprotein B100 ELISA Kit; Apolipoprotein B48 ELISA Kit; FLDB ELISA Kit; LDLCQ4 ELISA Kit
缩写：APOB
Uniprot No.：P04114
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, saliva, urine
检测范围：78 ng/mL-5000 ng/mL
灵敏度：19.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:800 | Average % | 104 | ||||
| Range % | 102-108 | |||||
| 1:1600 | Average % | 87 | ||||
| Range % | 81-92 | |||||
回收率：
| The recovery of human Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-102 | ||||
| EDTA plasma (n=4) | 90 | 87-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 50000.0740.0730.074 25000.1180.1210.120 12500.1820.1720.177 6250.2720.2840.278 3120.4510.4420.447 1560.7700.7540.762 781.1221.0541.088 02.3712.2702.321 | ||||||
货期：3-5 working days
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