首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠α1微球蛋白/bikunin蛋白前体(AMBP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AMBP含量。试剂盒检测范围为1.563 ng/mL-100 ng/mL，灵敏度为0.649 ng/mL。为科研领域研究AMBP在代谢性疾病模型、组织损伤修复机制或药物干预效果评估中的动态变化提供可靠工具，尤其适用于动物实验中的生物标志物探索及分子通路机制解析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ambp ELISA Kit; ItilProtein AMBP [Cleaved into: Alpha-1-microglobulin; Inter-alpha-trypsin inhibitor light chain ELISA Kit; ITI-LC ELISA Kit; Bikunin ELISA Kit; HI-30); Trypstatin] ELISA Kit
缩写：AMBP
Uniprot No.：Q64240
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.563 ng/mL-100 ng/mL
灵敏度：0.649 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 105 | |||||
| Range % | 99-109 | ||||||
| 1:200 | Average % | 96 | |||||
| Range % | 92-99 | ||||||
| 1:400 | Average % | 95 | |||||
| Range % | 90-98 | ||||||
| 1:800 | Average % | 89 | |||||
| Range % | 84-94 | ||||||
回收率：
| The recovery of rat AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 87-95 | |||||
| EDTA plasma (n=4) | 99 | 99-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 1000.1160.1240.120 500.2310.2450.238 250.4780.4980.488 12.51.0191.0251.022 6.251.4261.3671.397 3.1252.1592.1682.164 1.5632.4662.5442.505 02.8452.6542.750 | |||||||
货期：3-5 working days
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