首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人α1微球蛋白/bikunin蛋白前体(AMBP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的AMBP含量。AMBP（人α1微球蛋白/bikunin蛋白前体）是一种由肝脏合成的糖蛋白，具有多重生物学功能，包括调节炎症反应、蛋白酶抑制和细胞外基质稳定。其表达水平变化与肾脏疾病、慢性炎症及肿瘤微环境调控等病理过程密切相关，是重要的生物标志物。试剂盒检测范围为0.156 ng/mL-10 ng/mL，灵敏度为0.039 ng/mL。在科研领域广泛应用于探索肾脏功能损伤的生物标志物筛选、肝脏合成功能评估、泌尿系统疾病机制研究以及炎症相关疾病模型中蛋白动态变化的定量分析，尤其适用于跨样本类型的比较研究，为深入解析AMBP在生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A1M ELISA Kit; Alpha 1 microglobulin/bikunin precursor ELISA Kit; Alpha 1 microglycoprotein ELISA Kit; Alpha-1 microglycoprotein ELISA Kit; Alpha-1-microglobulin ELISA Kit; AMBP ELISA Kit; AMBP_HUMAN ELISA Kit; Bikunin ELISA Kit; Complex-forming glycoprotein heterogeneous in charge ELISA Kit; EDC1 ELISA Kit; Growth inhibiting protein 19 ELISA Kit; HCP ELISA Kit; HI-30 ELISA Kit; HI30 ELISA Kit; IATIL ELISA Kit; ITI ELISA Kit; ITI-LC ELISA Kit; ITIL ELISA Kit; Microglobulin (alpha 1) ELISA Kit; Protein AMBP ELISA Kit; Protein HC ELISA Kit; Trypstatin ELISA Kit; Uristatin ELISA Kit; Uronic-acid-rich protein ELISA Kit; UTI ELISA Kit
缩写：AMBP
Uniprot No.：P02760
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 91 | ||||
| Range % | 86-98 | |||||
| 1:2000 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:4000 | Average % | 85 | ||||
| Range % | 81-88 | |||||
| 1:8000 | Average % | 89 | ||||
| Range % | 83-96 | |||||
回收率：
| The recovery of human AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 87-102 | ||||
| EDTA plasma (n=4) | 96 | 91-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.8622.7932.8282.679 52.5312.5672.5492.400 2.52.0022.0542.0281.879 1.251.2961.3151.3061.157 0.6250.6430.6720.6580.509 0.3120.4410.4530.4470.298 0.1560.2920.3000.2960.147 00.1470.1510.149 | ||||||
货期：3-5 working days
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