Mouse Fetuin A ELISA Kit

中文名： 小鼠胎球蛋白A ELISAkit

货号: IPD-E14042m

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
小鼠胎球蛋白A酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AHSG含量。AHSG即α2-HS糖蛋白，是肝脏合成的血浆糖蛋白。研究显示它参与多种生理病理过程，如调节细胞生长、免疫反应及矿物质代谢等。其机制可能包括与细胞表面受体结合，影响信号通路传导，进而调节细胞的功能与行为。试剂盒检测范围为6.25 ng/mL-400 ng/mL，灵敏度为1.56 ng/mL。适用于科研领域探究小鼠模型中肝脏功能异常、糖脂代谢紊乱、心血管疾病或骨代谢相关机制。其宽泛的样本兼容性可支持血清/血浆样本的体液分析，或通过组织匀浆检测特定器官的蛋白表达差异，为构建疾病模型、评估干预效果提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Ahsg ELISA kit; FetuaAlpha-2-HS-glycoprotein ELISA kit; Countertrypin ELISA kit; Fetuin-A ELISA kit
缩写：
AHSG
Uniprot No.：
P29699
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
6.25 ng/mL-400 ng/mL
灵敏度：
1.56 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)52.62750.90051.35352.90149.72550.990SD0.0410.0350.0370.0540.0490.047CV(%)5.2164.5634.7936.8446.4996.120

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse fetuin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:10

Average %

Range %

87-103

1:20

Average %

Range %

84-105

1:40

Average %

Range %

83-103

1:80

Average %

Range %

82-107

回收率：

The recovery of mouse fetuin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
Heparin plasma (n=4)	91	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



ng/ml	OD1	OD2	Average	Corrected
400	2.657	2.704	2.681	2.547
200	1.905	2.002	1.954	1.820
100	1.259	1.279	1.269	1.135
50	0.714	0.758	0.736	0.602
25	0.426	0.442	0.434	0.300
12.5	0.361	0.348	0.355	0.221
6.25	0.257	0.244	0.251	0.117
0	0.134	0.133	0.134