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产品描述:人胎球蛋白A酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织培养上清液、组织匀浆、尿液样本中的AHSG含量。AHSG即α2-HS糖蛋白,是肝脏合成的血浆糖蛋白。在生理中它参与钙稳态等过程。研究机制方面,其在代谢、免疫等领域受关注,如可能影响胰岛素敏感性、参与炎症调节,在糖尿病、心血管疾病等病理进程中作用受科研人员持续探究。试剂盒检测范围为15.6 ng/mL-1000 ng/mL,灵敏度为3.9 ng/mL。科研人员可借助在基础医学研究中探索胎球蛋白A在代谢性疾病模型中的作用机制,或在心血管疾病动物实验中分析其表达动态,同时支持临床前药物研发阶段对生物标志物的定量评估。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:59 kDa bone sialic acid-containing protein ELISA Kit; A2HS ELISA Kit; Aa2-066 ELISA Kit; AHS ELISA Kit; Ahsg alpha-2-HS-glycoprotein ELISA Kit; Ahsg ELISA Kit; Alpha 2 HS Glycoprotein ELISA Kit; Alpha 2 Z globulin ELISA Kit; Alpha-2-HS-glycoprotein ELISA Kit; Alpha-2-HS-glycoprotein chain B ELISA Kit; Alpha-2-Z-globulin ELISA Kit; Asialofetuin ELISA Kit; Ba alpha 2 glycoprotein ELISA Kit; Ba-alpha-2-glycoprotein ELISA Kit; BSP ELISA Kit; Countertrypin ELISA Kit; Fetua ELISA Kit; FETUA_HUMAN ELISA Kit; Fetuin ; mouse; homolog of ELISA Kit; Fetuin A ELISA Kit; Fetuin-A ELISA Kit; Glycoprotein PP63 ELISA Kit; HSGA ELISA Kit; pp63 ELISA Kit
缩写:AHSG
Uniprot No.:P02765
种属:Homo sapiens (Human)
样本类型:serum, plasma, cell culture supernates, tissue homogenates, urine
检测范围:15.6 ng/mL-1000 ng/mL
灵敏度:3.9 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Metabolism
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human fetuin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1000 | Average % | 95 | ||||
Range % | 90-100 | |||||
1:2000 | Average % | 94 | ||||
Range % | 91-99 | |||||
1:4000 | Average % | 92 | ||||
Range % | 88-98 | |||||
1:8000 | Average % | 93 | ||||
Range % | 84-102 |
回收率:
The recovery of human fetuin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 97 | 92-106 | ||||
EDTA plasma (n=4) | 98 | 91-105 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected 10002.7542.7972.7762.564 5002.3122.3542.3332.121 2501.5631.5541.5591.347 1250.7350.7530.7440.532 62.50.5380.5260.5320.320 31.20.3220.3350.3290.117 15.60.2680.2840.2760.064 00.2110.2130.212 |
货期:3-5 working days
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