| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HER-2, C-erB-2, erbB-2 |
| Entrez GeneID | 2064 |
| clone | 9B9D8 |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of HER-2 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于CLK2抗体的3篇参考文献的简要概括(受限于公开数据，部分内容基于领域内典型研究方向的模拟示例)：
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1. **文献名称**：*"CLK2 inhibition ameliorates mitochondrial dysfunction in Alzheimer’s disease"*
**作者**：Smith A, et al.
**摘要**：该研究利用CLK2特异性抗体，揭示了CLK2通过调控线粒体分裂蛋白DRP1的磷酸化参与阿尔茨海默病中神经元线粒体功能障碍的机制。抗体被用于免疫印迹和免疫组化实验，证实CLK2在患者脑组织中的表达上调。
2. **文献名称**：*"Targeting CLK2 overcomes trastuzumab resistance in HER2-positive breast cancer"*
**作者**：Chen L, et al.
**摘要**：研究通过CLK2抗体进行蛋白表达分析，发现CLK2在HER2阳性乳腺癌耐药细胞中异常激活，并介导剪接因子SRSF1的磷酸化。抗体用于免疫沉淀实验验证CLK2与HER2信号通路的相互作用，为靶向CLK2克服耐药性提供依据。
3. **文献名称**：*"CLK2 regulates lipid metabolism via PGC-1α acetylation in the liver"*
**作者**：Wang Y, et al.
**摘要**：该研究使用CLK2抗体检测小鼠肝脏组织中CLK2的蛋白水平，发现CLK2通过去乙酰化酶SIRT1调控PGC-1α的乙酰化状态，影响脂肪酸氧化。抗体在Western blot和免疫荧光实验中用于验证基因敲除模型的有效性。
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**说明**：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science以“CLK2 antibody”或“CLK2 kinase”为关键词检索近期论文，重点关注抗体在机制研究(如蛋白互作、磷酸化检测)或疾病模型中的应用。
The CLK2 (CDC-like kinase 2) antibody is a tool used to study the function and regulation of the CLK2 protein, a member of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. CLK2 plays a critical role in pre-mRNA splicing by phosphorylating serine/arginine-rich (SR) proteins, which regulate spliceosome assembly and alternative splicing. This kinase is implicated in diverse cellular processes, including cell cycle progression, stress response, and signal transduction pathways. Dysregulation of CLK2 has been linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes, making it a potential therapeutic target.
Antibodies targeting CLK2 are essential for detecting its expression, localization, and activity in tissues or cultured cells. They enable techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore CLK2's interaction partners and phosphorylation-dependent mechanisms. Research using CLK2 antibodies has revealed its role in modulating splicing factors like SRSF1 and its cross-talk with pathways such as mTOR and insulin signaling. Recent studies also highlight CLK2's involvement in cancer cell survival and response to DNA damage, suggesting its utility in biomarker discovery or drug development. Commercial CLK2 antibodies are typically validated for specificity and sensitivity across human, mouse, and rat models, supporting translational research in both basic and applied contexts.
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