首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠聚集蛋白(AGRN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AGRN含量。AGRN即聚集蛋白，在神经肌肉接头形成过程中起关键作用。它是一种糖蛋白。研究机制方面，它能与肌肉上的受体结合，诱导乙酰胆碱受体聚集，对神经肌肉信号传递至关重要，相关研究有助于理解神经肌肉疾病机制。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。广泛应用于神经科学、肌肉发育及退行性病变机制研究，特别适用于动物模型中AGRN表达调控分析、药物干预效果评估及基础科研中蛋白功能探索，为神经肌肉系统相关疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Agrn ELISA kit; AgrinAgrin [Cleaved into: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment ELISA kit; C90); Agrin C-terminal 22 kDa fragment ELISA kit; C22)] ELISA kit
缩写：AGRN
Uniprot No.：P25304
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 86-91 | |||||
回收率：
| The recovery of rat AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 97-103 | ||||
| EDTA plasma (n=4) | 96 | 93-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 50002.1772.1982.1882.091 25001.8471.8631.8551.758 12501.2931.2731.2831.186 6250.8110.8460.8290.732 312.50.5050.4960.5010.404 1560.3010.3210.3110.214 780.1880.1890.1890.092 00.0960.0980.097 | ||||||
货期：3-5 working days
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