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产品描述：人脂联素(ADP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、尿液样本中的ADP含量。ADP（二磷酸腺苷）在细胞能量代谢中至关重要，是ATP水解供能后的产物。其研究机制方面，ADP参与血小板活化与聚集过程，与血栓形成相关。同时，它也涉及细胞内能量信号传导等过程，对其研究有助于开发抗血栓等相关药物。试剂盒检测范围为1.562 ng/mL-100 ng/mL，灵敏度为1.102 ng/mL。适用于肥胖症、糖尿病等代谢疾病机制研究，或药物干预模型中脂联素表达水平的动态监测，为体外实验提供高特异性检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：30 kDa adipocyte complement related protein ELISA Kit; 30 kDa adipocyte complement-related protein ELISA Kit; ACDC ELISA Kit; Acrp 30 ELISA Kit; ACRP30 ELISA Kit; ADIPO_HUMAN ELISA Kit; Adipocyte ELISA Kit; Adipocyte C1q and collagen domain containing protein ELISA Kit; Adipocyte complement related 30 kDa protein ELISA Kit; Adipocyte complement related protein of 30 kDa ELISA Kit; Adipocyte complement-related 30 kDa protein ELISA Kit; adipocyte-specific secretory protein ELISA Kit; Adiponectin ELISA Kit; Adiponectin precursor ELISA Kit; adiponectin; C1Q and collagen domain containing ELISA Kit; Adipoq ELISA Kit; Adipose most abundant gene transcript 1 ELISA Kit; Adipose most abundant gene transcript 1 protein ELISA Kit; Adipose specific collagen like factor ELISA Kit; ADIPQTL1 ELISA Kit; ADPN ELISA Kit; APM 1 ELISA Kit; apM-1 ELISA Kit; APM1 ELISA Kit; C1q and collagen domain-containing protein ELISA Kit; GBP 28 ELISA Kit; GBP28 ELISA Kit; Gelatin binding protein ELISA Kit; Gelatin binding protein 28 ELISA Kit; gelatin-binding protein 28 ELISA Kit; Gelatin-binding protein ELISA Kit; OTTHUMP00000210047 ELISA Kit
缩写：ADP
Uniprot No.：Q15848
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, urine
检测范围：1.562 ng/mL-100 ng/mL
灵敏度：1.102 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料：
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A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ADP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ADP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
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A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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