Rat Activin A,ACV-A ELISA Kit

中文名： 大鼠活化素A(ACV-A)ELISA Kit

货号: IPD-E04503r

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
大鼠活化素A(ACV-A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACV-A含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于研究ACV-A在生殖生物学、免疫调控机制、代谢性疾病模型中的表达动态，或用于评估药物干预、基因编辑等实验条件下ACV-A的分泌水平变化，为相关领域的分子机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
ACV-A
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 pg/mL-1000 pg/mL
灵敏度：
3.9 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)250.287251.377254.271255.277253.524253.271SD0.0290.0300.0340.0440.0420.045CV(%)3.764.614.836.526.467.08

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

87-93

1:2

Average %

101

Range %

96-108

1:4

Average %

Range %

84-91

1:8

Average %

100

Range %

95-105

回收率：

The recovery of rat ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	87	83-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.












pg/ml	OD1	OD2	Average	Corrected
1000	2.582	2.601	2.592	2.474
500	1.959	1.869	1.914	1.796
250	1.301	1.298	1.300	1.182
125	0.823	0.831	0.827	0.709
62.5	0.498	0.501	0.500	0.382
31.2	0.325	0.336	0.331	0.213
15.6	0.237	0.241	0.239	0.121
0	0.117	0.119	0.118