| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 9 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human APOC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DNASE1L1抗体的3篇文献示例(内容为模拟概括，非真实文献)：
1. **文献名称**: *"DNASE1L1 regulates mitochondrial DNA degradation during apoptosis"*
**作者**: Smith A, et al.
**摘要**: 本研究利用特异性DNASE1L1抗体，通过免疫印迹和免疫荧光技术，揭示了DNASE1L1在细胞凋亡过程中定位在线粒体并参与mtDNA降解的机制，证明其缺失导致凋亡延迟。
2. **文献名称**: *"Development of a monoclonal antibody targeting DNASE1L1 for neurodegenerative disease studies"*
**作者**: Chen L, et al.
**摘要**: 文章报道了一种新型高特异性DNASE1L1单克隆抗体的制备与验证，该抗体成功应用于阿尔茨海默病模型脑组织切片，证实DNASE1L1表达异常与神经元DNA损伤相关。
3. **文献名称**: *"DNASE1L1 suppresses tumorigenesis by preventing cytoplasmic DNA accumulation"*
**作者**: Wang X, et al.
**摘要**: 通过DNASE1L1抗体进行功能阻断实验，研究发现DNASE1L1通过水解胞质DNA抑制炎症信号通路，其表达下调与多种癌症的基因组不稳定性显著相关。
(注：以上为模拟内容，实际文献需通过PubMed/Google Scholar等平台检索确认。)
The DNASE1L1 antibody is a tool used to detect deoxyribonuclease 1-like 1 (DNASE1L1), a calcium-dependent endonuclease implicated in DNA fragmentation during apoptosis. DNASE1L1. also known as DNase γ, shares structural homology with DNASE1 (DNase I) but exhibits distinct substrate preferences and cellular localization. It is encoded by the *DNASE1L1* gene and expressed in tissues like the liver, kidney, and immune cells. Unlike DNASE1. which is secreted, DNASE1L1 localizes intracellularly, potentially within mitochondria or nuclei, suggesting roles in mitochondrial DNA degradation or chromatin breakdown.
Research using DNASE1L1 antibodies has explored its involvement in apoptotic pathways, autoimmune diseases, and cancer. Dysregulation of DNASE1L1 is linked to impaired DNA clearance, which may trigger autoimmune responses (e.g., systemic lupus erythematosus) due to accumulation of self-DNA. In cancer, altered DNASE1L1 expression correlates with tumor progression or chemoresistance. The antibody aids in studying its expression patterns, post-translational modifications, and interactions with apoptotic regulators like Bcl-2 family proteins.
Commercial DNASE1L1 antibodies are typically validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence. Challenges include cross-reactivity with related DNases, necessitating careful validation via knockout controls. Ongoing studies aim to clarify its precise biological functions and therapeutic potential as a biomarker or drug target.
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