| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CFD1; NBP 2; NUBP1 |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NUBP2抗体的3篇示例参考文献(注：文献为虚拟示例，建议通过学术数据库检索真实研究)：
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1. **文献名称**: *NUBP2 regulates mitotic progression through interaction with the cytoskeleton*
**作者**: Smith J, et al. (2016)
**摘要**: 本研究利用NUBP2抗体进行免疫印迹和免疫荧光实验，发现NUBP2通过调控微管动力学参与有丝分裂进程，敲低NUBP2导致染色体排列异常。
2. **文献名称**: *NUBP2 modulates iron-sulfur cluster biogenesis in human cells*
**作者**: Zhang L, et al. (2018)
**摘要**: 通过NUBP2抗体的免疫共沉淀(Co-IP)实验，揭示NUBP2与线粒体铁硫簇组装蛋白ISCU的相互作用，证实其在细胞铁代谢中的关键作用。
3. **文献名称**: *Overexpression of NUBP2 correlates with poor prognosis in colorectal cancer*
**作者**: Tanaka K, et al. (2020)
**摘要**: 采用NUBP2抗体对结直肠癌组织进行免疫组化分析，发现NUBP2高表达与肿瘤转移及患者生存率降低显著相关，提示其作为癌症标志物的潜力。
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建议通过PubMed或Google Scholar以关键词“NUBP2 antibody”、“NUBP2 function”检索最新研究。
The NUBP2 (Nucleotide-Binding Protein 2) antibody is a tool used to study the NUBP2 protein, a member of the cytosolic iron-sulfur cluster assembly (CIA) machinery. NUBP2. along with its homolog NUBP1. forms a heterotetrameric complex critical for the maturation and transfer of iron-sulfur (Fe-S) clusters to target apoproteins, which are essential for diverse cellular processes, including DNA repair, enzyme catalysis, and mitochondrial function. Dysregulation of NUBP2 has been implicated in diseases such as cancer and neurodegenerative disorders, where Fe-S cluster biogenesis is compromised.
The NUBP2 antibody is typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. It is widely employed in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect NUBP2 expression levels, localization, and interactions in cell lines or tissues. Researchers use this antibody to explore NUBP2's role in cellular proliferation, redox homeostasis, and mitochondrial dynamics. Specificity is validated via knockout/knockdown controls or siRNA experiments. Commercial NUBP2 antibodies are often accompanied by validation data (e.g., species reactivity, application suitability) to ensure reproducibility. Studies utilizing this antibody have contributed to understanding molecular mechanisms underlying cancer progression, particularly in contexts where Fe-S cluster deficiency drives genomic instability or metabolic reprogramming.
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