| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | BOR; DasraB; MESRGP; BOREALIN |
| WB Predicted band size | 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CDCA8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CDCA8抗体的3篇参考文献示例(注：部分内容基于文献主题概括，建议通过学术数据库核实完整信息)：
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1. **文献名称**：*Borealin: A novel chromosomal passenger complex subunit required for kinetochore targeting*
**作者**：Gassmann, R., Carvalho, A., Henzing, A.J., et al.
**摘要**：该研究阐明了CDCA8(Borealin)作为染色体乘客复合体(CPC)的关键组分，在调控有丝分裂中姐妹染色单体分离和微管-动粒结合中的核心作用。作者通过抗体阻断实验，证实CDCA8缺失会导致染色体排列异常和细胞周期停滞。
2. **文献名称**：*CDCA8 overexpression predicts poor prognosis in hepatocellular carcinoma and promotes tumor cell proliferation*
**作者**：Li, Y., Wang, X., Zhang, H., et al.
**摘要**：研究利用CDCA8抗体进行免疫组化分析，发现肝癌组织中CDCA8蛋白高表达与患者预后不良显著相关。体外实验表明，CDCA8通过激活Aurora B激酶通路驱动癌细胞增殖，提示其作为潜在治疗靶点。
3. **文献名称**：*Development of a monoclonal antibody specific to CDCA8 for functional studies in mitosis*
**作者**：Tanaka, H., Abe, S., Watanabe, T.
**摘要**：本文报道了一种高特异性抗CDCA8单克隆抗体的开发与验证。该抗体成功应用于免疫荧光和Western blot，揭示了CDCA8在细胞周期不同阶段的动态定位变化，为研究其功能提供了可靠工具。
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如需具体文献全文，建议通过PubMed或Google Scholar搜索标题或作者获取DOI链接。
CDCA8 (Cell Division Cycle Associated 8), also known as Borealin, is a key component of the chromosomal passenger complex (CPC), which plays a vital role in regulating mitosis and cytokinesis. The CPC, comprising CDCA8. Survivin, INCENP, and Aurora B kinase, ensures proper chromosome segregation, spindle assembly checkpoint signaling, and correction of microtubule-kinetochore attachment errors. CDCA8 specifically contributes to CPC localization and stability, facilitating its recruitment to centromeres during prophase and transfer to the spindle midzone during anaphase. Dysregulation of CDCA8 is linked to genomic instability, mitotic defects, and cancer progression.
CDCA8 antibodies are essential tools for studying its expression, localization, and functional mechanisms in cellular processes. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate CDCA8's role in cell cycle regulation, cancer biology, and therapeutic targeting. Overexpression of CDCA8 has been observed in various cancers (e.g., breast, colorectal, and lung cancers), correlating with poor prognosis and chemoresistance. Researchers also utilize CDCA8 antibodies to explore its potential as a biomarker for cancer diagnosis or as a target for small-molecule inhibitors in anticancer therapies. Developing high-specificity CDCA8 antibodies remains critical for advancing mechanistic studies and clinical applications.
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