| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | LAMPB; CD107b; LAMP-2; LGP110 |
| WB Predicted band size | 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human LAMP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LAMP2抗体的3-4篇参考文献及其简要摘要：
1. **"LAMP-2 deficiency leads to impaired autophagy and Danon disease"**
- **作者**: Tanaka Y, et al.
- **摘要**: 该研究揭示了LAMP2蛋白在自噬过程中的关键作用，LAMP2基因突变导致Danon病(一种罕见的溶酶体贮积症)，表现为心肌病和骨骼肌异常。研究通过小鼠模型证明LAMP2缺失会导致自噬体与溶酶体融合障碍。
2. **"LAMP2 regulates autophagy in the mammalian heart"**
- **作者**: Nishino I, et al.
- **摘要**: 本文通过LAMP2抗体在心脏组织中的免疫染色分析，发现LAMP2缺失会导致心脏细胞自噬功能异常，进一步阐明了其在心脏病理中的重要性，并与人类Danon病的临床表现相关联。
3. **"Antibody-based profiling of lysosomal membrane proteins in human tissues"**
- **作者**: Eskelinen EL, et al.
- **摘要**: 研究开发了针对溶酶体膜蛋白(包括LAMP2)的特异性抗体，用于免疫印迹和免疫组化分析，验证了LAMP2在多种组织中的表达模式及其在溶酶体功能研究中的应用价值。
4. **"Role of LAMP2 in lysosomal biogenesis and pathogen clearance"**
- **作者**: Saftig P, et al.
- **摘要**: 该文献利用LAMP2抗体研究其在溶酶体成熟和病原体降解中的作用，发现LAMP2缺失会损害溶酶体酸化过程，影响细胞内病原体(如细菌)的清除能力。
这些文献涵盖了LAMP2的分子功能、疾病机制及实验工具应用，可作为相关研究的核心参考。
LAMP2 (Lysosomal-Associated Membrane Protein 2) is a key transmembrane glycoprotein predominantly located in lysosomal and late endosomal membranes. It belongs to the LAMP family, which includes LAMP1 and LAMP2. both critical for maintaining lysosomal structural integrity and function. LAMP2 consists of two domains: a heavily glycosylated luminal domain, which shields the lysosomal membrane from degradation, and a short cytoplasmic tail involved in intracellular trafficking and organelle interactions.
This protein plays multifaceted roles in autophagy, lysosomal enzyme activity regulation, and antigen presentation. Notably, LAMP2 is essential for autophagosome-lysosome fusion during autophagy, a cellular waste clearance process. Three splice variants (LAMP2A, LAMP2B, LAMP2C) exist, with LAMP2A specifically linked to chaperone-mediated autophagy.
Mutations in the LAMP2 gene are associated with Danon disease, a rare X-linked disorder characterized by cardiomyopathy, skeletal myopathy, and intellectual disability. LAMP2 antibodies are widely used in research to study lysosomal dynamics, autophagy-related mechanisms, and disease pathology. They also aid in diagnosing lysosomal storage disorders and monitoring protein expression in cancers or neurodegenerative diseases. Commercial LAMP2 antibodies typically target conserved regions of the luminal domain, enabling applications in Western blot, immunofluorescence, and immunohistochemistry.
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