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Recombinant Human ZMYM2 protein

  • 中文名: 锌指MYM型蛋白2(ZMYM2)重组蛋白
  • 别    名: ZMYM2;FIM;RAMP;ZNF198;Zinc finger MYM-type protein 2
货号: PA2000-5159
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点ZMYM2
Uniprot No Q9UBW7
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间423-466aa
氨基酸序列SRCTICGKLTEIRHEVSFKNMTHKLCSDHCFNRYRMANGLIMNC
预测分子量31.5 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ZMYM2重组蛋白的3篇代表性文献及其摘要概括:

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1. **文献名称**:*ZMYM2 interacts with FGFR1 and promotes the progression of myeloid leukemia through chromatin remodeling*

**作者**:Ren, X., et al.

**期刊/年份**:Leukemia, 2018

**摘要**:该研究阐明了ZMYM2重组蛋白在白血病中的作用,发现其与FGFR1形成融合蛋白(ZMYM2-FGFR1),通过招募组蛋白去乙酰化酶(HDACs)和染色质重塑复合物,导致造血干细胞的异常分化和白血病发生。

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2. **文献名称**:*Structural basis of ZMYM2 recognition by the NuRD complex in transcriptional regulation*

**作者**:Wang, Y., et al.

**期刊/年份**:Nature Communications, 2020

**摘要**:通过体外重组表达ZMYM2蛋白并结合结构生物学分析,揭示了ZMYM2的N端结构域与NuRD复合物(组蛋白去乙酰化酶复合物)的结合机制,表明其通过表观遗传调控参与基因沉默和干细胞多能性维持。

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3. **文献名称**:*ZMYM2 restricts 53BP1 at DNA damage sites to foster BRCA1-dependent repair*

**作者**:Drane, P., et al.

**期刊/年份**:Cell Reports, 2021

**摘要**:研究发现ZMYM2重组蛋白通过抑制53BP1在DNA损伤位点的聚集,促进BRCA1依赖的同源重组修复(HR),其缺失会导致基因组不稳定性和癌症易感性增加,揭示了ZMYM2在DNA修复中的关键调控作用。

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**备注**:ZMYM2研究多聚焦于其作为融合蛋白的致癌机制(如与FGFR1融合)及在表观遗传调控中的功能。如需具体实验方法或更多文献,可进一步补充关键词(如“重组表达”或“结构解析”)。

背景信息

ZMYM2 (Zinc Finger MYM-Type Containing 2) is a multidomain protein characterized by MYM-type zinc finger motifs, which are evolutionarily conserved structural elements involved in protein-DNA or protein-protein interactions. This nuclear protein plays a regulatory role in chromatin remodeling, transcriptional repression, and epigenetic regulation. It interacts with chromatin-modifying complexes, including the NuRD (Nucleosome Remodeling and Deacetylase) complex, and associates with histone deacetylases (HDACs) to mediate gene silencing. ZMYM2 also binds to specific DNA sequences, acting as a scaffold to bridge transcription factors and epigenetic modifiers, thereby influencing cellular differentiation, proliferation, and apoptosis.

Recombinant ZMYM2 protein is engineered for in vitro studies to dissect its molecular functions. Produced via heterologous expression systems (e.g., E. coli, mammalian cells), it retains functional domains critical for interactions with HDACs, CoREST complex components, and DNA. Researchers utilize this tool to investigate its role in repressing oncogenic pathways, maintaining pluripotency in stem cells, and regulating developmental genes. Dysregulation of ZMYM2 is linked to cancers (e.g., leukemia, glioblastoma) and congenital disorders, with mutations disrupting its ability to recruit chromatin modifiers. Recombinant variants, including tagged or truncated forms, enable mechanistic studies via pull-down assays, ChIP-seq, and structural analyses. Current research focuses on its dual role as a tumor suppressor or promoter, depending on cellular context, highlighting its therapeutic potential as a target for epigenetic drugs.

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