Recombinant VACWR052 protein

中文名： 包膜磷脂酶OPG057(VACWR052)重组蛋白
别名： VACWR052；Envelope phospholipase OPG057

货号: PA2000-5085

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	VACWR052
Uniprot No	P04021
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-372aa
氨基酸序列	MWPFASVPAGAKCRLVETLPENMDFRSDHLTTFECFNEIITLAKKYIYIASFCCNPLSTTRGALIFDKLKEASEKGIKIIVLLDERGKRNLGELQSHCPDINFITVNIDKKNNVGLLLGCFWVSDDERCYVGNASFTGGSIHTIKTLGVYSDYPPLATDLRRRFDTFKAFNSAKNSWLNLCSAACCLPVSTAYHIKNPIGGVFFTDSPEHLLGYSRDLDTDVVIDKLKSAKTSIDIEHLAIVPTTRVDGNSYYWPDIYNSIIEAAINRGVKIRLLVGNWDKNDVYSMATARSLDALCVQNDLSVKVFTIQNNTKLLIVDDEYVHITSANFDGTHYQNHGFVSFNSIDKQLVSEAKKIFERDWVSSHSKSLKI
预测分子量	49.2 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于VACWR052重组蛋白的模拟参考文献示例(注：实际文献可能需要通过学术数据库验证，以下为虚构示例)：

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1. **标题**：*Expression and Characterization of Recombinant VACWR052 Protein in Mammalian Cells*

**作者**：Smith J, Lee T, et al.

**摘要**：本研究成功克隆并在哺乳动物细胞中表达了痘苗病毒(Vaccinia virus)Western Reserve株的VACWR052重组蛋白，证实其可通过亲和层析高效纯化。实验表明，该蛋白在体外具有免疫原性，可能作为疫苗开发的潜在靶点。

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2. **标题**：*Functional Analysis of VACWR052 in Viral Replication*

**作者**：Garcia R, Patel S, et al.

**摘要**：通过敲除VACWR052基因的痘苗病毒突变体，研究发现该蛋白在病毒复制周期中起调控作用。重组VACWR052的补充实验表明，其可能参与病毒粒子组装，影响宿主细胞的免疫逃逸机制。

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3. **标题**：*Structural Insights into VACWR052 and Its Interaction with Host Proteins*

**作者**：Wang L, Kim H, et al.

**摘要**：利用X射线晶体学解析了VACWR052重组蛋白的三维结构，揭示了其与宿主细胞NF-κB通路关键蛋白的结合位点。研究为开发靶向该蛋白的抗病毒药物提供了理论依据。

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4. **标题**：*VACWR052 Recombinant Protein as a Novel Vaccine Adjuvant*

**作者**：Müller F, Zhang Y, et al.

**摘要**：在小鼠模型中评估VACWR052重组蛋白的佐剂效应，发现其可增强抗原特异性抗体和T细胞反应，提示其在联合疫苗设计中的潜在应用价值。

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建议通过PubMed、Web of Science或Google Scholar检索真实文献(关键词：VACWR052/Vaccinia virus WR052/recombinant protein)，或查阅痘苗病毒相关蛋白质功能研究的综述。

背景信息

VACWR052 is a recombinant protein derived from the vaccinia virus (VACV), a member of the *Poxviridae* family historically used in smallpox vaccines. The VACV Western Reserve (WR) strain, known for its robust replication and broad host range, serves as the genetic backbone for this protein. VACWR052 is encoded by the WR052 gene, which is conserved among orthopoxviruses and hypothesized to play roles in immune evasion or viral-host interactions. Its precise biological function remains under investigation, but studies suggest potential involvement in modulating host immune responses, such as interfering with innate signaling pathways (e.g., Toll-like receptors or interferon responses) to facilitate viral persistence.

Recombinant VACWR052 is typically expressed in heterologous systems (e.g., *E. coli* or mammalian cells) for structural and functional studies. Its production enables detailed characterization of antigenic properties, interactions with host proteins, and mechanisms underlying immune regulation. Researchers are particularly interested in its application as a tool for vaccine development, either as an immunogen to elicit targeted immune responses or as a component of vectored vaccines to deliver foreign antigens. Additionally, its role in viral pathogenesis makes it a candidate for antiviral drug design or immunotherapy strategies against poxvirus infections.

The protein’s structure, though not fully resolved, is predicted to include conserved domains typical of poxviral immunomodulators. Ongoing research aims to clarify its contribution to viral fitness and host adaptation, with implications for improving vaccine safety and understanding viral immune escape. As a recombinant product, VACWR052 bridges virology and biotechnology, offering insights into both fundamental poxvirus biology and applied biomedical innovations.