| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TSG10; VPS23 |
| Entrez GeneID | 7251 |
| clone | 8E10D10 |
| WB Predicted band size | 44kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TSG101 (AA: 167-374) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于TSG101抗体的参考文献摘要(基于经典研究，具体文献信息可能存在年份差异)：
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1. **文献名称**: *ESCRT-I proteins and ubiquitin cooperate to mediate ESCRT-III recruitment to membranes*
**作者**: Katzmann DJ, Babst M, Emr SD
**摘要**: 该研究利用TSG101抗体进行免疫共沉淀实验，揭示了ESCRT-I复合体(含TSG101)通过识别泛素化信号招募ESCRT-III至内体膜，调控多泡体形成和膜重塑的分子机制。
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2. **文献名称**: *Late domain function of HIV-1 gp41 requires interaction with TSG101*
**作者**: Demirov DG, Freed EO
**摘要**: 通过TSG101抗体阻断实验，证明HIV-1病毒颗粒出芽依赖其衣壳蛋白gp41的"late domain"与TSG101的直接结合，揭示了TSG101在病毒出芽中的关键作用。
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3. **文献名称**: *TSG101 aberrations in human breast cancer*
**作者**: Oh H, et al.
**摘要**: 采用TSG101抗体进行免疫组化和Western blot分析，发现乳腺癌组织中TSG101蛋白表达异常(缺失或截短)，提示其作为肿瘤抑制因子的潜在功能失调与癌症进展相关。
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**备注**：若需具体抗体开发或应用文献，建议补充TSG101抗体货号或研究场景(如WB/IF等)，以便进一步筛选。
TSG101 (tumor susceptibility gene 101) antibody is a widely used tool in biomedical research targeting the TSG101 protein, a key component of the endosomal sorting complex required for transport (ESCRT) machinery. Discovered in 1996 through studies linking its dysfunction to tumorigenesis, TSG101 plays critical roles in membrane remodeling, vesicle trafficking, and protein sorting, particularly in multivesicular body (MVB) formation, viral budding, and cytokinesis. It is essential for HIV-1 release, exosome secretion, and cellular processes like autophagy and cell cycle regulation.
TSG101 antibodies are primarily employed to detect and quantify TSG101 expression in techniques such as Western blotting, immunofluorescence, and immunoprecipitation. Researchers use these antibodies to study cancer biology, neurodegenerative diseases (e.g., Alzheimer’s), viral pathogenesis, and extracellular vesicle dynamics. Commercial TSG101 antibodies are often generated against specific epitopes (e.g., human TSG101 amino acids 1-150) and validated across species like humans, mice, and rats. Common clones include 4A10 and C2.
Due to TSG101's functional diversity, its antibodies help dissect molecular mechanisms in membrane scission, receptor degradation, and cell division. However, specificity validation (e.g., knockout cell controls) is crucial, as off-target binding has been reported. Major suppliers include Cell Signaling Technology, Santa Cruz Biotechnology, and Abcam, each offering antibodies optimized for distinct applications.
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