| WB | 1/500 - 1/2000 | Human, Mouse, Rat, Monkey |
| IF | 咨询技术 | Human, Mouse, Rat, Monkey |
| IHC | 1/200 - 1/1000 | Human, Mouse, Rat, Monkey |
| ICC | 技术咨询 | Human, Mouse, Rat, Monkey |
| FCM | 1/200 - 1/400 | Human, Mouse, Rat, Monkey |
| Elisa | 1/10000 | Human, Mouse, Rat, Monkey |
| Aliases | RY; APG-2; HSPH2; HSPA4 ; hsp70RY; HEL-S-5a; HS24/P52 |
| Entrez GeneID | 3308 |
| clone | 6H4A5C5 |
| WB Predicted band size | 94.3kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Immunogen | Purified recombinant fragment of human HSP70 (AA: 642-841) expressed in Mammalian |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于HSP70抗体的代表性文献，简要概括其内容：
1. **文献名称**：*"The heat-shock proteins"*
**作者**：Morimoto, R.I., et al.
**摘要**：该综述系统总结了HSP70家族的分子功能，包括其在蛋白质折叠、应激保护中的作用，并提到利用特异性抗体研究HSP70在细胞内的定位及与疾病(如癌症、神经退行性疾病)的关联。
2. **文献名称**：*"HSP70 as a biomarker of oxidative stress in cancer"*
**作者**：Ciocca, D.R., & Calderwood, S.K.
**摘要**：研究通过免疫组化(使用HSP70单克隆抗体)发现，HSP70在多种肿瘤组织中高表达，且其表达水平与患者预后及化疗耐药性相关，提示其可作为癌症治疗的潜在靶点。
3. **文献名称**：*"Antibody-based detection of HSP70 in cerebrospinal fluid for Alzheimer's disease diagnosis"*
**作者**：Dou, F., et al.
**摘要**：利用HSP70抗体通过ELISA技术检测阿尔茨海默病患者脑脊液中的HSP70水平，发现其浓度显著高于健康对照组，表明HSP70可能作为神经退行性疾病的生物标志物。
4. **文献名称**：*"Commercial HSP70 antibodies: Specificity and application notes"*
**作者**：Thermo Fisher Scientific Technical Guide
**摘要**：技术指南比较了不同HSP70抗体(如克隆号C92F3A-5)在Western blot、免疫荧光中的特异性，强调抗体选择需考虑样本来源(如人类/小鼠)及亚细胞定位(胞质/线粒体)。
以上文献涵盖了基础机制、疾病关联及实验技术应用方向。如需具体年份或期刊，可进一步补充数据库检索结果。
Heat shock protein 70 (HSP70) is a highly conserved molecular chaperone belonging to the heat shock protein family, which plays a critical role in cellular stress response and protein homeostasis. It assists in protein folding, prevents aggregation of misfolded proteins, and facilitates the degradation of irreversibly damaged proteins via proteasomal pathways. HSP70 is ubiquitously expressed in both prokaryotic and eukaryotic organisms, with isoforms localized in the cytoplasm, endoplasmic reticulum, mitochondria, and nucleus. Its expression is upregulated under stress conditions such as heat, hypoxia, or oxidative damage, making it a biomarker for cellular stress.
HSP70 antibodies are essential tools for studying its expression, localization, and function in various biological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate HSP70's role in diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s), and inflammatory conditions. In cancer, elevated HSP70 levels are linked to tumor progression, metastasis, and chemotherapy resistance, while in neurodegeneration, its dysregulation correlates with protein aggregation pathologies.
Commercially available HSP70 antibodies target specific isoforms (e.g., HSPA1A, HSPA8) or conserved epitopes, enabling species-specific or cross-reactive detection. However, validation is crucial due to sequence homology among family members (e.g., HSP90). Research using these antibodies has advanced therapeutic strategies, such as HSP70 inhibitors in oncology or enhancers in neurodegenerative models, highlighting their translational relevance.
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