| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TRYP2 |
| Entrez GeneID | 3003 |
| clone | 2F9C12 |
| WB Predicted band size | 29kda |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GZMK (AA: 27-264) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于GZMK抗体的3篇示例文献摘要(注：以下内容为模拟参考，建议通过学术数据库核实真实文献)：
1. **文献名称**: "Granzyme K: A novel mediator of CD8+ T cell-mediated cytotoxicity"
**作者**: Hansen K. et al.
**摘要**: 研究利用GZMK特异性抗体发现，GZMK在活化的CD8+ T细胞中高表达，并通过非凋亡途径促进靶细胞死亡，提示其在抗病毒免疫中的作用。
2. **文献名称**: "Granzyme K modulates TLR signaling to control pro-inflammatory cytokine production"
**作者**: Andrade F. et al.
**摘要**: 通过抗GZMK抗体阻断实验，揭示GZMK通过调节TLR信号通路影响巨噬细胞炎症因子释放，与脓毒症病理相关。
3. **文献名称**: "Elevated granzyme K expression in tumor-infiltrating lymphocytes predicts poor prognosis in hepatocellular carcinoma"
**作者**: Saito M. et al.
**摘要**: 使用GZMK抗体对肝癌组织进行免疫组化分析，发现高表达GZMK的T细胞浸润与患者生存率下降相关，提示其促肿瘤进展机制。
4. **文献名称**: "Autoantibodies against granzyme K in rheumatoid arthritis"
**作者**: Zhang L. et al.
**摘要**: 通过ELISA检测患者血清中的抗GZMK自身抗体，发现其水平与类风湿性关节炎活动性正相关，可能参与自身免疫损伤。
建议通过PubMed或Google Scholar搜索关键词“GZMK antibody”或“Granzyme K function”获取真实文献。
**Background of GZMK Antibody**
GZMK (Granzyme K) is a serine protease predominantly expressed in cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is stored in cytotoxic granules and released upon immune cell activation to eliminate infected or malignant cells. Unlike other granzymes (e.g., GZMB), GZMK exhibits distinct substrate specificity and plays dual roles in immune regulation. While it can induce caspase-independent cell death, recent studies highlight its proinflammatory functions, such as cleaving extracellular matrix proteins and activating cytokines, contributing to immune cell recruitment and tissue inflammation.
GZMK antibodies are essential tools for studying its expression, localization, and function in immune responses. They are widely used in techniques like flow cytometry, Western blotting, and immunohistochemistry to investigate GZMK's role in diseases, including viral infections, cancer, and autoimmune disorders. For instance, elevated GZMK levels are linked to chronic inflammation in conditions like rheumatoid arthritis and COVID-19. suggesting its potential as a therapeutic target or biomarker.
Research utilizing GZMK antibodies has also uncovered its non-cytotoxic roles, such as modulating cytokine signaling and extracellular proteolysis, broadening understanding of granzyme biology beyond traditional cell-killing mechanisms. These antibodies thus serve as critical reagents for dissecting GZMK's contributions to both protective immunity and pathological inflammation.
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