| WB | 1/500 - 1/2000 | Human, Monkey |
| IF | 咨询技术 | Human, Monkey |
| IHC | 咨询技术 | Human, Monkey |
| ICC | 1/200 - 1/1000 | Human, Monkey |
| FCM | 1/200 - 1/400 | Human, Monkey |
| Elisa | 1/10000 | Human, Monkey |
| Aliases | ADIR2; NUP96; NUP196; Nup98-96 |
| Entrez GeneID | 4928 |
| clone | 3G8C7 |
| WB Predicted band size | 197.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Monkey |
| Immunogen | Purified recombinant fragment of human NUP98 (AA: 1-218) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于NUP98抗体的参考文献及其摘要概括：
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1. **文献名称**：*NUP98-HOXA9 induces long-term proliferation and blocks differentiation of primary human CD34+ hematopoietic cells*
**作者**：Takeda A, et al.
**摘要**：研究利用NUP98抗体通过免疫沉淀和Western blot技术，验证NUP98-HOXA9融合蛋白在白血病中的致癌机制，发现其通过阻断造血干细胞分化并激活增殖信号通路驱动白血病发生。
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2. **文献名称**：*NUP98 fusion proteins interact with the MLL complex to drive leukemogenesis*
**作者**：Kasper LH, et al.
**摘要**：通过NUP98抗体进行染色质免疫共沉淀(ChIP)实验，揭示NUP98融合蛋白与MLL复合物的相互作用，阐明其在急性髓系白血病(AML)中通过表观遗传调控异常激活致癌基因的机制。
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3. **文献名称**：*Role of NUP98 in nuclear pore complex assembly and mRNA export*
**作者**：Xu S, Powers MA
**摘要**：利用NUP98特异性抗体进行免疫荧光和电镜分析，发现NUP98在核孔复合体(NPC)组装中的关键作用，并证明其C端结构域对mRNA出核转运的调控功能。
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**备注**：上述文献为示例，实际引用时建议通过PubMed或Web of Science以“NUP98 antibody”为关键词检索近期研究，优先选择高影响力期刊(如*Blood*、*Leukemia*等)中涉及抗体应用(如染色、IP、功能研究)的论文。
The NUP98 antibody is a crucial tool in studying the nucleoporin 98 (NUP98) protein, a component of the nuclear pore complex (NPC) that regulates nucleocytoplasmic transport. NUP98 plays roles in mRNA export, gene expression, and chromatin organization. It is frequently implicated in hematologic malignancies, particularly acute myeloid leukemia (AML), where chromosomal translocations generate NUP98 fusion oncoproteins (e.g., NUP98-HOXA9). Antibodies targeting NUP98 are essential for detecting these fusion proteins, aiding in disease diagnosis and mechanistic research.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to localize NUP98 within nuclear speckles or NPCs. They also help investigate NUP98’s interaction partners, such as transcriptional regulators or viral proteins (e.g., HIV-1 integrase), and its role in developmental processes using model organisms.
Research applications extend to studying NUP98’s FG-repeat domains, which mediate transport receptor interactions, and its involvement in cellular stress responses or viral replication. Commercially available NUP98 antibodies vary in specificity (monoclonal vs. polyclonal) and are validated for cross-reactivity across species (human, mouse, rat). However, challenges persist due to homologous regions in fusion proteins or paralogs like NUP96. Recent studies also explore NUP98’s non-canonical roles in mitosis and DNA repair, expanding its relevance in cancer biology and therapeutics.
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