| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Dlst |
| Uniprot No | P36957 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453aa |
| 氨基酸序列 | MLSRSRCVSRAFSRSLSAFQKGNCPLGRRSLPGVSLCQGPGYPNSRKVVINNSVFSVRFFRTTAVCKDDLVTVKTPAFAESVTEGDVRWEKAVGDTVAEDEVVCEIETDKTSVQVPSPANGVIEALLVPDGGKVEGGTPLFTLRKTGAAPAKAKPAEAPAAAAPKAEPTAAAVPPPAAPIPTQMPPVPSPSQPPSGKPVSAVKPTVAPPLAEPGAGKGLRSEHREKMNRMRQRIAQRLKEAQNTCAMLTTFNEIDMSNIQEMRARHKEAFLKKHNLKLGFMSAFVKASAFALQEQPVVNAVIDDTTKEVVYRDYIDISVAVATPRGLVVPVIRNVEAMNFADIERTITELGEKARKNELAIEDMDGGTFTISNGGVFGSLFGTPIINPPQSAILGMHGIFDRPVAIGGKVEVRPMMYVALTYDHRLIDGREAVTFLRKIKAAVEDPRVLLLDL |
| 预测分子量 | 48.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇模拟的关于DLST(Dihydrolipoamide S-succinyltransferase)重组蛋白研究的参考文献示例(注：以下内容为示例性虚构，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"High-yield expression and purification of recombinant human DLST in Escherichia coli for functional studies"*
**作者**: Zhang L, et al.
**摘要**: 本研究报道了通过大肠杆菌表达系统高效表达人源DLST重组蛋白的优化策略，采用His标签亲和层析纯化，并验证了重组蛋白的酶活性及其在三羧酸循环中的功能。
2. **文献名称**: *"Structural characterization of DLST in mitochondrial 2-oxoglutarate dehydrogenase complex"*
**作者**: Tanaka K, et al.
**摘要**: 通过哺乳动物细胞表达系统获得DLST重组蛋白，结合冷冻电镜技术解析其与2-酮戊二酸脱氢酶复合物的相互作用，揭示了DLST在能量代谢中的结构调控机制。
3. **文献名称**: *"DLST deficiency rescue by recombinant protein delivery: Implications in metabolic disorders"*
**作者**: Chen R, et al.
**摘要**: 开发了基于HEK293细胞的DLST重组蛋白生产平台，证明外源性重组DLST可恢复缺陷型细胞的线粒体功能，为代谢疾病的蛋白替代疗法提供实验依据。
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如需真实文献，建议通过PubMed、Web of Science或Google Scholar搜索关键词："DLST recombinant protein expression"或"Dihydrolipoamide S-succinyltransferase purification"。
Dlst (dihydrolipoamide S-acetyltransferase) is a critical enzyme component of the mitochondrial pyruvate dehydrogenase complex (PDC), which plays a central role in cellular energy metabolism. As part of PDC, Dlst catalyzes the transfer of an acetyl group from pyruvate to coenzyme A, linking glycolysis to the tricarboxylic acid (TCA) cycle. This process is essential for converting glucose-derived pyruvate into acetyl-CoA, a key substrate for ATP production. Dysregulation of Dlst function has been implicated in metabolic disorders, neurodegenerative diseases, and cancer, highlighting its physiological and pathological significance.
Recombinant Dlst protein is produced using genetic engineering techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to ensure proper folding and post-translational modifications. Its recombinant form enables detailed biochemical characterization, including studies on enzyme kinetics, protein-protein interactions within PDC, and responses to metabolic regulators. Researchers utilize recombinant Dlst to investigate mitochondrial dysfunction mechanisms, screen potential therapeutic compounds targeting metabolic pathways, and develop diagnostic tools for diseases associated with PDC deficiencies.
Structural studies of recombinant Dlst have provided insights into its catalytic mechanism and regulatory domains, facilitating drug design efforts. Additionally, it serves as a valuable reagent for metabolic pathway reconstitution in synthetic biology applications. The development of recombinant Dlst has significantly advanced our understanding of cellular energetics and disease pathophysiology, offering potential avenues for therapeutic intervention in diabetes, cancer, and rare inherited metabolic syndromes.
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