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Recombinant Human MT-ATP6 protein

  • 中文名: ATP合酶亚基a(MT-ATP6)重组蛋白
  • 别    名: MT-ATP6;ATP6;ATPASE6;MTATP6;ATP synthase subunit a
货号: PA2000-4851
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点MT-ATP6
Uniprot NoP00846
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-226
氨基酸序列MNENLFASFIAPTILGLPAAVLIILFPPLLIPTSKYLINNRLITTQQWLIKLTSKQMMTMHNTKGRTWSLMLVSLIIFIATTNLLGLLPHSFTPTTQLSMNLAMAIPLWAGTVIMGFRSKIKNALAHFLPQGTPTPLIPMLVIIETISLLIQPMALAVRLTANITAGHLLMHLIGSATLAMSTINLPSTLIIFTILILLTILEIAVALIQAYVFTLLVSLYLHDNT
预测分子量24.8kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于MT-ATP6重组蛋白的3篇参考文献及其摘要概括:

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1. **文献名称**: *Functional analysis of mitochondrial ATP synthase subunit 6 mutations in yeast*

**作者**: Kucharczyk R, et al.

**摘要**: 研究通过酵母模型表达重组人源MT-ATP6蛋白,验证其在线粒体ATP酶复合体组装中的作用,并分析Leber遗传性视神经病变相关突变的致病机制,发现特定突变导致质子通道功能异常。

2. **文献名称**: *Recombinant expression and purification of human mitochondrial ATP synthase subunit 6 in E. coli*

**作者**: Smith J, et al.

**摘要**: 报道了利用大肠杆菌系统成功表达并纯化重组MT-ATP6蛋白,优化了跨膜结构域的溶解条件,为后续结构分析和药物靶点研究提供技术基础。

3. **文献名称**: *Mitochondrial ATP6 mutations alter cellular energy metabolism and oxidative stress response*

**作者**: Garcia-Sanchez A, et al.

**摘要**: 通过哺乳动物细胞系表达突变型MT-ATP6重组蛋白,揭示其导致ATP合成效率下降、活性氧(ROS)水平升高,并引发细胞凋亡,解释了其在神经退行性疾病中的作用。

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注:上述文献为示例,实际研究中建议通过PubMed或Web of Science检索最新论文。若需具体文章,可提供更详细的研究方向(如疾病关联或结构解析)。

背景信息

**Background of MT-ATP6 Recombinant Protein**

MT-ATP6 is a mitochondrial gene-encoded subunit of ATP synthase (Complex V), a critical enzyme in oxidative phosphorylation (OXPHOS) responsible for generating adenosine triphosphate (ATP), the primary energy currency of cells. This 226-amino acid protein forms part of the FO transmembrane domain of ATP synthase, contributing to proton translocation across the mitochondrial inner membrane, which drives ATP synthesis. Mutations in MT-ATP6 are linked to mitochondrial disorders, such as Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa (NARP), underscoring its role in cellular energy metabolism.

Recombinant MT-ATP6 protein is produced using heterologous expression systems (e.g., *E. coli* or yeast) to enable detailed biochemical and structural studies. Its production involves cloning the MT-ATP6 gene into expression vectors, followed by protein purification via affinity chromatography. Challenges include ensuring proper folding and membrane integration, as MT-ATP6 is a hydrophobic, multi-pass transmembrane protein.

Research on recombinant MT-ATP6 aids in elucidating mechanisms of ATP synthase dysfunction in mitochondrial diseases, drug screening, and developing gene therapies. It also serves as a tool to study proton transport dynamics, structure-function relationships, and the impact of pathogenic mutations. By providing a controlled source of the protein, recombinant MT-ATP6 facilitates advancements in understanding mitochondrial bioenergetics and therapeutic interventions for energy metabolism disorders.

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