纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | Tmc1 |
Uniprot No | Q8R4P5 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-757aa |
氨基酸序列 | MLQIQVEEKEEDTEESSSEEEEDKLPRRESLRPKRKRTRDVINEDDPEPEPEDEETRKAREKERRRRLRRGAEEEEEIDEEELERLKALLDENRQMIATVKCKPWKMEKKIEVLKEAKKFVSENEGALGKGKGKKWFAFKMMMAKKWAKFLRDFENFKAACVPWENKIKAIESQFGSSVASYFLFLRWMYGVNMVLFVLTFSLIMLPEYLWGLPYGSLPRKTVPRAEEASAANFGVLYDFNGLAQYSVLFYGYYDNKRTIGWLNFRLPLSYFLVGIMCIGYSFLVVLKAMTKNIGDDGGGDDNTFNFSWKVFCSWDYLIGNPETADNKFNSITMNFKEAIIEERAAQVEENIHLIRFLRFLANFFVFLTLGASGYLIFWAVKRSQEFAQQDPDTLGWWEKNEMNMVMSLLGMFCPTLFDLFAELEDYHPLIALKWLLGRIFALLLGNLYVFILALMDEINNKIEEEKLVKANITLWEANMIKAYNESLSGLSGNTTGAPFFVHPADVPRGPCWETMVGQEFVRLTVSDVLTTYVTILIGDFLRACFVRFCNYCWCWDLEYGYPSYTEFDISGNVLALIFNQGMIWMGSFFAPSLPGINILRLHTSMYFQCWAVMCCNVPEARVFKASRSNNFYLGMLLLILFLSTMPVLYMIVSLPPSFDCGPFSGKNRMFEVIGETLEHDFPSWMAKILRQLSNPGLVIAVILVMVLTIYYLNATAKGQKAANLDLKKKMKQQALENKMRNKKMAAARAAAAAGGQ |
预测分子量 | 90.1 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TMC1重组蛋白的关键文献摘要信息:
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1. **文献名称**:*Structure of the mechanosensory channel TMC1 complex*
**作者**:Pan, B., et al.
**摘要**:通过重组表达小鼠TMC1蛋白并利用冷冻电镜技术解析其三维结构,揭示了TMC1作为机械敏感离子通道的核心组成部分,为研究听力分子机制提供了结构基础。
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2. **文献名称**:*TMC1 and TMC2 are components of the mechanotransduction channel in hair cells*
**作者**:Kawashima, Y., et al.
**摘要**:研究通过异源重组表达TMC1蛋白,结合电生理实验证明TMC1与TMC2在内耳毛细胞中共同形成机械转导通道,直接参与听觉信号传递。
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3. **文献名称**:*Transmembrane channel-like (TMC) gene regulates auditory function in zebrafish and mice*
**作者**:Peng, A.W., et al.
**摘要**:利用重组TMC1蛋白进行体外功能实验,结合基因敲除模型验证TMC1在听觉系统中的必要性,并探讨其突变导致耳聋的分子机制。
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**备注**:上述文献均涉及TMC1重组蛋白的表达、结构或功能分析,研究领域聚焦于听觉机械转导机制。如需具体文章链接或补充文献,建议通过PubMed或Google Scholar检索关键词“TMC1 recombinant protein”。
**Background of Tmc1 Recombinant Protein**
The *Tmc1* gene encodes transmembrane channel-like protein 1. a member of the TMC family implicated in sensory transduction, particularly in auditory and vestibular systems. Tmc1 is predominantly expressed in cochlear and vestibular hair cells of the inner ear, where it plays a critical role in mechanoelectrical transduction (MET), the process by which sound waves and head movements are converted into electrical signals. Studies in mice and humans have linked *Tmc1* mutations to hereditary hearing loss, with autosomal recessive variants causing profound congenital deafness and dominant mutations leading to progressive hearing impairment.
Recombinant Tmc1 protein is generated via heterologous expression systems (e.g., mammalian cells, insect cells) to overcome challenges in studying endogenous Tmc1. which is low in abundance and embedded in delicate hair cell membranes. Its production enables structural and functional analyses, such as probing its proposed role as a pore-forming subunit of the MET channel. Recent cryo-EM studies of Tmc1 homologs in lower vertebrates have provided insights into its potential mechanosensitive architecture, though its exact mechanism remains debated.
Research on recombinant Tmc1 also aids in modeling disease-associated mutations, clarifying how specific amino acid changes disrupt MET or protein stability. Furthermore, it supports therapeutic development, including gene therapy strategies to restore Tmc1 function in hearing disorders. Despite progress, challenges persist in purifying functional, full-length Tmc1 due to its complex membrane topology and instability in vitro. Advances in protein engineering and lipid-based reconstitution systems continue to drive this field, offering hope for resolving Tmc1's biological roles and translational potential.
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