| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/500 - 1/2000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | K18; CK-18; CYK18 |
| Entrez GeneID | 3875 |
| clone | 6G12F2 |
| WB Predicted band size | 48kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human KRT18 (AA: 1-150) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于KRT18抗体的3篇参考文献示例(文献信息为模拟内容，仅供参考)：
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1. **文献名称**：*KRT18 as a Biomarker for Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma*
**作者**：Zhang Y, et al.
**摘要**：本研究通过免疫组化分析发现，KRT18抗体在肝细胞癌组织中的表达水平与上皮-间质转化(EMT)进程密切相关，提示其可作为肝癌转移潜力的潜在标志物。
2. **文献名称**：*Development of a Novel Monoclonal Antibody Against Cleaved KRT18 for Apoptosis Detection*
**作者**：Lee S, et al.
**摘要**：作者开发了一种特异性识别KRT18凋亡裂解片段(M30抗原)的单克隆抗体，验证了其在血清和细胞裂解液中检测肿瘤细胞凋亡的灵敏性及临床应用价值。
3. **文献名称**：*KRT18 Expression in Circulating Tumor Cells: Prognostic Significance in Breast Cancer*
**作者**：Wang H, et al.
**摘要**：研究利用KRT18抗体检测外周血循环肿瘤细胞(CTCs)，发现KRT18阳性CTCs与乳腺癌患者预后不良显著相关，为个体化治疗提供了依据。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“KRT18 antibody”、“KRT18 biomarker”或“KRT18 apoptosis”获取。
**Background of KRT18 Antibody**
Keratin 18 (KRT18), a type I intermediate filament protein, is primarily expressed in single-layered epithelial cells (e.g., liver, gastrointestinal tract) and glandular tissues. As a key component of the cytoskeleton, it pairs with Keratin 8 (KRT8) to form filaments that maintain cellular integrity, regulate mechanical stress responses, and participate in cell signaling. KRT18 antibodies are essential tools for detecting this protein in research and diagnostics.
In pathology, KRT18 is a biomarker for epithelial-derived cancers (e.g., carcinomas). Its expression patterns help differentiate tumor subtypes, while cleavage by caspases during apoptosis generates neoepitopes (e.g., M30 antigen), detectable via specific KRT18 antibodies to assess cell death. These antibodies are widely used in techniques like immunohistochemistry (IHC), Western blotting, and ELISA. For instance, the M30 antibody detects apoptosis-specific KRT18 fragments, aiding in monitoring chemotherapy response.
KRT18 mutations or aberrant expression are linked to liver diseases (e.g., Mallory-Denk bodies in alcoholic hepatitis) and genetic disorders. Antibodies against phosphorylated KRT18 (e.g., at Ser52) also study stress-induced keratin reorganization.
Variability in antibody clones (e.g., DC10. KS1A3) requires validation for specificity, as epitope differences impact detection in formalin-fixed or frozen samples. Overall, KRT18 antibodies are vital for understanding epithelial biology, disease mechanisms, and developing diagnostic or therapeutic strategies.
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