| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | APG4B; AUTL1 |
| Entrez GeneID | 23192 |
| clone | 4H9A5 |
| WB Predicted band size | 44.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ATG4B (AA: 1-221) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ATG4B抗体的示例参考文献(部分为虚构示例，供格式参考)：
1. **文献名称**：ATG4B regulates autophagic flux and modulates cancer cell survival
**作者**：Li, Y., et al.
**摘要**：该研究利用特异性ATG4B抗体，揭示了ATG4B在自噬体成熟中的核心作用，并发现其在多种癌症中高表达。实验表明，抑制ATG4B可增强化疗药物对肿瘤细胞的杀伤效果，提示其作为潜在治疗靶点。
2. **文献名称**：Development and validation of a novel monoclonal antibody for human ATG4B detection
**作者**：Smith, J., et al.
**摘要**：报道了一种高特异性ATG4B单克隆抗体的开发，通过免疫印迹和免疫荧光验证其在细胞系及组织样本中的应用，并证明该抗体可有效检测自噬过程中ATG4B的活性变化。
3. **文献名称**：ATG4B phosphorylation modulates its proteolytic activity in neurodegenerative disease models
**作者**：Chen, L., et al.
**摘要**：研究通过ATG4B抗体发现其磷酸化修饰(如AKT介导的Ser383位点)可抑制LC3切割活性，导致自噬缺陷，该机制在阿尔茨海默病模型中与病理蛋白聚集相关。
4. **文献名称**：ATG4B as a biomarker for autophagy dysfunction in metabolic disorders
**作者**：Wang, H., et al.
**摘要**：利用ATG4B抗体分析肥胖患者肝脏组织样本，发现ATG4B表达水平与自噬通量降低及胰岛素抵抗显著相关，提示其可作为代谢性疾病的新型生物标志物。
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注：以上文献为示例性内容，实际研究请通过PubMed或Google Scholar以“ATG4B antibody”为关键词检索最新论文。
ATG4B, a member of the ATG4 cysteine protease family, is a critical enzyme in autophagy, a conserved cellular degradation process. It regulates autophagosome formation by processing microtubule-associated protein 1 light chain 3 (LC3), a key autophagy marker. ATG4B cleaves the C-terminal region of pro-LC3 to generate cytosolic LC3-I, which is subsequently conjugated to phosphatidylethanolamine (PE) to form LC3-II for autophagosome membrane anchoring. Additionally, ATG4B delipidates LC3-II from autophagosomal membranes to recycle LC3 during autophagy completion. Dysregulation of ATG4B activity is implicated in various diseases, including cancer and neurodegenerative disorders, due to its role in balancing autophagy flux.
ATG4B antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect ATG4B protein levels in cells or tissues. Researchers also employ these antibodies to investigate post-translational modifications or interactions with autophagy-related proteins. Since ATG4B activity is modulated by oxidative stress and inhibitors like cystatin, specific antibodies help evaluate its enzymatic state in experimental models. Understanding ATG4B's role through antibody-based assays contributes to exploring therapeutic strategies targeting autophagy pathways in disease contexts.
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