Mouse Monoclonal NFKB2 Antibody

Nuclear factor kappa B subunit 2 (NFKB2) is a member of the NF-κB transcription factor family, which regulates immune responses, inflammation, cell survival, and differentiation. The NFKB2 gene encodes a precursor protein, p100. that undergoes partial proteasomal processing to generate the mature p52 subunit. This processing is tightly controlled and mediates activation of the non-canonical NF-κB signaling pathway. NFKB2 antibodies are essential tools for studying the expression, localization, and post-translational modifications of NFKB2/p100/p52 in various biological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate NFKB2's role in diseases such as cancers, autoimmune disorders, and immunodeficiency syndromes (e.g., COMMAD syndrome). Specific NFKB2 antibodies may target distinct regions of the protein, such as the N-terminus (p52-specific) or the C-terminus (p100-specific), enabling researchers to differentiate between precursor and processed forms. Dysregulation of NFKB2 is linked to abnormal B-cell development, chronic inflammation, and tumor progression, making its detection critical for mechanistic studies. Validation of antibody specificity, often via knockout cell lines or siRNA knockdown, is crucial due to structural similarities with other NF-κB family members like NFKB1 (p105/p50). Commercial NFKB2 antibodies are typically raised in rabbits or mice, with applications spanning basic research and clinical diagnostics.