Recombinant Human HMGA2 protein

中文名： 高迁移率族AT Hook蛋白2(HMGA2)重组蛋白
别名： HMGA2;HMGIC;High mobility group protein HMGI-C

货号: PA1000-1462

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>85%SDS-PAGE.
种属	Human
靶点	HMGA2
Uniprot No	P52926
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-109aa
氨基酸序列	MSARGEGAGQ PSTSAQGQPA APAPQKRGRG RPRKQQQEPT GEPSPKRPRG RPKGSKNKSP SKAAQKKAEA TGEKRPRGRP RKWPQQVVQK KPAQEETEET SSQESAEEDL EHHHHHH
预测分子量	13 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于HMGA2重组蛋白的参考文献示例，按您的要求整理：

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1. **文献名称**：*HMGA2 Recombinant Protein Binds to DNA and Modulates Gene Expression in Cancer Cells*

**作者**：Cleynen I, et al.

**摘要**：研究通过体外表达纯化HMGA2重组蛋白，分析其与DNA特定序列的结合能力，并证明其在癌症细胞中通过调控靶基因(如CCND2)促进细胞增殖和侵袭。

2. **文献名称**：*Expression and Functional Analysis of Recombinant HMGA2 in Epithelial-Mesenchymal Transition*

**作者**：Schuldenfrei A, et al.

**摘要**：利用大肠杆菌系统表达HMGA2重组蛋白，发现其通过激活TGF-β通路诱导上皮细胞间质转化(EMT)，揭示了其在肿瘤转移中的分子机制。

3. **文献名称**：*Structural Characterization of HMGA2 Recombinant Protein and Its Role in Chromatin Remodeling*

**作者**：Resar LMS, et al.

**摘要**：通过核磁共振(NMR)解析HMGA2重组蛋白的AT-hook结构域构象，阐明其如何通过改变染色质结构调控基因转录，尤其在干细胞分化中的关键作用。

4. **文献名称**：*HMGA2 Recombinant Protein Promotes Adipogenesis by Regulating PPARγ Expression*

**作者**：Zhou X, et al.

**摘要**：研究发现HMGA2重组蛋白通过直接结合PPARγ基因启动子区，促进脂肪细胞分化，为肥胖相关代谢疾病提供了新的分子靶点。

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注：以上文献信息为示例，实际引用时需根据具体研究补充完整信息(期刊、年份、卷号等)。如需真实文献，建议在PubMed或Web of Science中检索关键词“HMGA2 recombinant protein”。

背景信息

HMGA2 (High Mobility Group AT-hook 2) is a non-histone chromatin-associated protein belonging to the HMGA family, known for its role in regulating gene expression by modulating chromatin structure. It contains three conserved AT-hook DNA-binding domains that enable interaction with AT-rich regions of DNA, facilitating the assembly of transcriptional complexes. HMGA2 is implicated in embryonic development, cellular differentiation, and processes like proliferation and apoptosis. Dysregulation of HMGA2. particularly its overexpression, is frequently associated with cancer progression, metastasis, and poor prognosis, making it a potential therapeutic target.

Recombinant HMGA2 protein is engineered for in vitro studies to elucidate its molecular mechanisms. Produced using heterologous expression systems (e.g., E. coli, mammalian cells), the recombinant protein retains functional domains while enabling controlled experimental conditions. Its production typically involves cloning the HMGA2 coding sequence into expression vectors, followed by purification via affinity tags (e.g., His-tag, GST-tag). Researchers utilize recombinant HMGA2 to investigate DNA-binding properties, protein-protein interactions, and its role in epigenetic regulation or oncogenic signaling pathways. It also serves as a tool for screening inhibitors or studying post-translational modifications (e.g., phosphorylation) that modulate its activity. Validated through techniques like Western blot, EMSA, or mass spectrometry, recombinant HMGA2 provides a standardized reagent for mechanistic and translational studies in cancer biology and beyond.