| WB | 1/500 - 1/2000 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 1/200 - 1/1000 | Human,Monkey |
| ICC | 技术咨询 | Human,Monkey |
| FCM | 1/200 - 1/400 | Human,Monkey |
| Elisa | 1/10000 | Human,Monkey |
| Aliases | GE; NE; HLE; HNE; ELA2; SCN1; PMN-E |
| Entrez GeneID | 1991 |
| clone | 6B6B10 |
| WB Predicted band size | 28.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human ELANE (AA: 140-267) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4条关于ELANE抗体的参考文献示例(内容基于公开研究概括，非真实文献)：
1. **文献名称**：*ELANE Antibodies as Diagnostic Markers in Congenital Neutropenia*
**作者**：Smith A, et al. (2018)
**摘要**：研究探讨ELANE抗体在先天性中性粒细胞减少症(CN)中的诊断价值，发现ELANE蛋白异常表达与患者中性粒细胞功能缺陷相关，提示其可作为CN的潜在生物标志物。
2. **文献名称**：*Neutrophil Elastase (ELANE) in Chronic Inflammation: Role of Specific Antibodies in Disease Monitoring*
**作者**：Johnson R, et al. (2020)
**摘要**：通过ELANE抗体检测慢性炎症性疾病患者的中性粒细胞弹性蛋白酶水平，发现其浓度与疾病活动度正相关，为治疗评估提供依据。
3. **文献名称**：*Targeting ELANE with Neutralizing Antibodies Attenuates Lung Injury in Mouse Models*
**作者**：Lee C, et al. (2021)
**摘要**：在小鼠模型中，使用ELANE中和抗体显著降低中性粒细胞介导的肺组织损伤，表明其作为急性呼吸窘迫综合征(ARDS)的治疗潜力。
4. **文献名称**：*ELANE Expression and Antibody-Based Detection in Pancreatic Cancer Progression*
**作者**：Brown K, et al. (2022)
**摘要**：研究ELANE在胰腺癌微环境中的表达，通过特异性抗体验证其与肿瘤相关中性粒细胞浸润及患者预后的关联，支持其在癌症免疫研究中的应用。
(注：以上文献为示例性概括，实际研究需通过数据库如PubMed、Google Scholar检索真实文献。)
The ELANE antibody targets neutrophil elastase (NE), a serine protease encoded by the *ELANE* gene (formerly *ELA2*). Primarily expressed in neutrophils, NE plays a critical role in innate immunity by degrading extracellular matrix components and microbial proteins during infection and inflammation. Dysregulation of NE activity is linked to several pathologies. For instance, mutations in *ELANE* cause severe congenital neutropenia (SCN) and cyclic neutropenia (CyN), autosomal dominant disorders characterized by recurrent infections due to low neutrophil counts. Additionally, excessive NE activity, often due to alpha-1-antitrypsin deficiency, contributes to tissue damage in chronic inflammatory conditions like emphysema and cystic fibrosis.
ELANE antibodies are essential tools in research and diagnostics. They enable the detection of NE expression, localization, and activity in biological samples, aiding studies on neutrophil function and inflammatory diseases. In clinical contexts, these antibodies help identify genetic mutations in SCN/CyN patients and assess NE levels in conditions like acute myeloid leukemia (AML) or sepsis. Recent studies also explore NE's role in cancer progression, particularly in tumor microenvironment modulation.
Understanding NE's dual role—as a defender against pathogens and a mediator of tissue injury—highlights the therapeutic potential of targeting ELANE. Inhibitors and monoclonal antibodies against NE are under investigation for treating inflammatory and genetic disorders. Thus, ELANE antibodies serve as pivotal reagents in unraveling neutrophil biology and advancing precision medicine approaches.
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