| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100 - 1/500 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MRD6; NR2B; hNR3; EIEE27; GluN2B; NMDAR2B |
| Entrez GeneID | 2904 |
| clone | 6E9A8 |
| WB Predicted band size | 166.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GRIN2B (AA: extra 27-163) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4篇关于GRIN2B抗体的参考文献及其简要摘要：
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1. **文献名称**: "GRIN2B encephalopathy: Novel findings on phenotype, variant clustering, functional consequences and antibody interactions"
**作者**: Myers et al.
**摘要**: 研究分析了GRIN2B基因突变引起的神经发育障碍，验证了特异性抗体在患者细胞模型中的表达检测，发现部分突变导致NMDA受体功能异常，并探讨抗体在突变蛋白结合中的应用。
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2. **文献名称**: "Mechanism and regulation of NMDA receptor activation by GRIN2B-specific antibodies"
**作者**: Swanger et al.
**摘要**: 通过体外实验验证了多种GRIN2B抗体的特异性，发现某些抗体可选择性识别受体亚基构象变化，为研究NMDA受体动态调控提供了工具，并用于阿尔茨海默病模型中的蛋白定位。
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3. **文献名称**: "GRIN2B mutations in autism spectrum disorder: Functional validation using antibody-based assays"
**作者**: O’Roak et al.
**摘要**: 利用GRIN2B抗体对自闭症患者携带的基因突变进行功能验证，发现部分突变导致受体表面表达减少，抗体检测结果与电生理数据关联，支持其在疾病机制研究中的价值。
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4. **文献名称**: "Antibody-based profiling of synaptic NMDA receptor composition in neurological disorders"
**作者**: Köhr & Monyer
**摘要**: 开发了一种基于GRIN2B抗体的免疫印迹和免疫荧光方法，定量分析人脑组织样本中NMDA受体亚基表达变化，揭示其在精神分裂症和癫痫中的潜在病理作用。
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这些文献涵盖了GRIN2B抗体在基因突变分析、疾病模型研究、受体功能探索及诊断工具开发中的应用，反映了其在神经科学领域的多样性和重要性。
The GRIN2B antibody targets the protein encoded by the GRIN2B gene, which is a critical subunit (GluN2B) of the N-methyl-D-aspartate (NMDA) receptor, a type of ionotropic glutamate receptor. NMDA receptors are essential for synaptic plasticity, learning, and memory. The GluN2B subunit determines receptor biophysical properties, localization, and downstream signaling. GRIN2B antibodies are widely used in neuroscience research to study the expression, distribution, and function of NMDA receptors in the central nervous system. They are employed in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect GluN2B in tissues or cultured cells, aiding investigations into receptor complex assembly and synaptic activity regulation.
Mutations in GRIN2B are linked to neurodevelopmental disorders, including intellectual disability, autism spectrum disorder, and epilepsy. Research using GRIN2B antibodies has helped elucidate how altered receptor function contributes to these conditions. Additionally, these antibodies are valuable in studying neurological diseases like Alzheimer's and stroke, where NMDA receptor dysregulation is implicated. Challenges in using GRIN2B antibodies include ensuring specificity due to sequence homology with other NMDA receptor subunits. Validation via knockout controls or peptide blocking is critical. Overall, GRIN2B antibodies are indispensable tools for exploring NMDA receptor biology and its role in health and disease.
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