| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100 - 1/500 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | A2aR; RDC8; ADORA2 |
| Entrez GeneID | 135 |
| clone | 6A6G11 |
| WB Predicted band size | 44.7kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human ADORA2A (AA: 274-412) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是3篇与ADORA2A抗体相关的参考文献示例(注：以下为模拟示例，实际文献需通过学术数据库核实)：
1. **文献名称**：*Immunohistochemical localization of adenosine A2A receptors in the rat central nervous system*
**作者**：Rosin DL, et al.
**摘要**：本研究通过ADORA2A特异性抗体，在大鼠中枢神经系统中定位A2A受体的分布，发现其高表达于纹状体、嗅结节等区域，为研究其在运动调节中的作用提供依据。
2. **文献名称**：*Adenosine A2A receptor upregulation in human lymphocytes during chronic caffeine consumption*
**作者**：Varani K, et al.
**摘要**：利用ADORA2A抗体进行流式细胞术和Western blot分析，发现长期咖啡因摄入可上调人淋巴细胞中A2A受体的表达，提示其与免疫调节的关联性。
3. **文献名称**：*Selective blockade of ADORA2A receptors by monoclonal antibody attenuates neuroinflammation in Parkinson's disease models*
**作者**：Chen JF, et al.
**摘要**：开发针对ADORA2A的单克隆抗体，并在帕金森病动物模型中验证其抗神经炎症效果，证明其通过抑制小胶质细胞活化减轻多巴胺能神经元损伤。
4. **文献名称**：*Validation of commercial anti-ADORA2A antibodies for protein quantification in human brain tissue*
**作者**：Schwarzschild MA, et al.
**摘要**：系统性评估市售ADORA2A抗体的特异性和灵敏度，确认其在人脑组织Western blot及免疫荧光中的可靠性，为临床研究提供标准化工具。
The adenosine A2a receptor (ADORA2A) is a G protein-coupled receptor (GPCR) that binds adenosine, a purine nucleoside involved in cellular energy regulation and signaling. Primarily expressed in the central nervous system (particularly the basal ganglia), immune cells, and cardiovascular tissues, ADORA2A modulates neurotransmission, inflammation, and blood flow. It plays a key role in dopamine signaling pathways, making it a therapeutic target for neurological disorders like Parkinson’s disease. In immunology, ADORA2A activation suppresses inflammatory responses, influencing conditions such as cancer and autoimmune diseases.
ADORA2A antibodies are tools used to detect and study the receptor’s expression, localization, and function in research. These antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes on the receptor’s extracellular or intracellular domains. Applications include Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Validated antibodies help investigate ADORA2A’s role in cAMP signaling, receptor trafficking, and interactions with ligands or drugs. Specificity and cross-reactivity (e.g., with other adenosine receptor subtypes like ADORA1 or ADORA2B) are critical validation steps. In drug development, ADORA2A antibodies aid in evaluating antagonists/agonists for therapies targeting neuroprotection, immune modulation, or cardiovascular regulation. Research using these antibodies has advanced understanding of adenosine-mediated pathways in both physiological and pathological contexts.
×
