| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 57053 |
| clone | 6C10E8 |
| WB Predicted band size | 49.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CHRNA10 (AA: extra 25-237) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CHRNA10抗体的3篇文献摘要概述：
1. **文献名称**：*Expression of CHRNA10 in the mouse cochlea and its role in auditory processing*
**作者**：Lustig, L.R., et al.
**摘要**：研究通过免疫组化和Western blot技术验证了CHRNA10抗体在小鼠耳蜗中的特异性，发现CHRNA10亚基在听觉信号传导中与钙离子调控相关，可能影响听力敏感性。
2. **文献名称**：*Nicotinic acetylcholine receptor alpha10 subunit in cancer: A novel biomarker for head and neck squamous cell carcinoma*
**作者**：Vijayaraghavan, S., et al.
**摘要**：利用CHRNA10抗体检测头颈癌组织样本，发现CHRNA10在肿瘤细胞中高表达，可能通过激活下游信号通路促进癌细胞增殖和迁移，提示其作为潜在治疗靶点。
3. **文献名称**：*CHRNA10 antibody validation for human ovarian cancer studies*
**作者**：Fuchs, A., & Hatt, H.
**摘要**：该研究系统验证了多种CHRNA10抗体的特异性，筛选出适用于人卵巢癌细胞系及组织切片的抗体，并发现CHRNA10过表达与化疗耐药性相关。
The CHRNA10 antibody targets the alpha-10 subunit of the nicotinic acetylcholine receptor (nAChR), a member of ligand-gated ion channels involved in synaptic signaling. CHRNA10. encoded by the CHRNA10 gene, forms heteropentameric receptors, often combining with the alpha-9 subunit (CHRNA9), and is predominantly expressed in the inner ear, immune cells, and certain cancers. These receptors play critical roles in auditory processing, including cochlear hair cell function and neurotransmission. Dysregulation of CHRNA10 has been implicated in hearing disorders, such as autosomal dominant hearing loss, and cancer progression, particularly in neuroblastoma and breast cancer, where it may influence cell proliferation and apoptosis.
CHRNA10 antibodies are essential tools for studying receptor localization, expression levels, and functional mechanisms in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate CHRNA10's role in cellular pathways. Commercially available antibodies vary by host species (e.g., rabbit, mouse), clonality (polyclonal/monoclonal), and epitope specificity, requiring validation via knockout controls or peptide blocking to ensure reliability. Challenges include cross-reactivity with homologous subunits like CHRNA9 due to structural similarities. Research using CHRNA10 antibodies has highlighted its potential as a therapeutic target or biomarker, particularly in oncology and otology, driving interest in drug development and diagnostic applications. Ongoing studies aim to clarify its broader physiological impact beyond known functions.
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