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Recombinant Human HK3 protein

  • 中文名: 己糖激酶3(HK3)重组蛋白
  • 别    名: HK3;Hexokinase-3
货号: PA1000-1438
Price: ¥询价
数量:
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产品详情

纯度>95%SDS-PAGE.
种属Human
靶点HK3
Uniprot NoP52790
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-923aa
氨基酸序列MDSIGSSGLRQGEETLSCSEEGLPGPSDSSELVQECLQQFKVTRAQLQQI QASLLGSMEQALRGQASPAPAVRMLPTYVGSTPHGTEQGDFVVLELGATG ASLRVLWVTLTGIEGHRVEPRSQEFVIPQEVMLGAGQQLFDFAAHCLSEF LDAQPVNKQGLQLGFSFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQ LLRDAIRRQGAYNIDVVAVVNDTVGTMMGCEPGVRPCEVGLVVDTGTNAC YMEEARHVAVLDEDRGRVCVSVEWGSFSDDGALGPVLTTFDHTLDHESLN PGAQRFEKMIGGLYLGELVRLVLAH LARCGVLFGGCTSPALLSQGSILLEHVAEMEDPSTGAARVHAILQDLGLS PGASDVELVQHVCAAVCTRAAQLCAAALAAVLSCLQHSREQQTLQVAVAT GGRVCERHPRFCSVLQGTVMLLAPECDVSLIPSVDGGGRGVAMVTAVAAR LAAHRRLLEETLAPFRLNHDQLAAVQAQMRKAMAKGLRGEASSLRMLPTF VRATPDGSERGDFLALDLGGTNFRVLLVRVTTGVQITSEIYSIPETVAQG SGQQLFDHIVDCIVDFQQKQGLSGQSLPLGFTFSFPCRQLGLDQGILLNW TKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYED PRCEIGLIVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLA MLSTRFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFR GQQIQRLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLPLTSDDALMV LEVCQAVSQRAAQLCGAGVAAVVEKIRENRGLEELAVSVGVDGTLYKLHP RFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVACRLAQLTRVLE HHHHHH
预测分子量100 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于HK3(己糖激酶3)重组蛋白的3篇示例文献摘要(注:文献信息为示例,建议通过学术数据库查询真实研究):

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1. **文献名称**:*Recombinant Hexokinase 3 (HK3) promotes glycolysis and cell proliferation in colorectal cancer*

**作者**:Zhang Y, et al.

**摘要**:本研究通过在大肠杆菌中表达并纯化人源HK3重组蛋白,探讨其在结直肠癌细胞糖代谢中的作用。实验表明,重组HK3通过增强糖酵解活性促进癌细胞增殖,提示其作为潜在治疗靶点的可能性。

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2. **文献名称**:*Expression and characterization of human HK3 in a mammalian cell system: Implications for mitochondrial dysfunction*

**作者**:Smith JL, et al.

**摘要**:利用哺乳动物表达系统成功获得高活性HK3重组蛋白,发现其在线粒体膜结合后调控细胞凋亡。研究揭示了HK3在代谢应激条件下对线粒体功能的保护作用。

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3. **文献名称**:*Structural analysis of recombinant HK3 reveals isoform-specific substrate binding sites*

**作者**:Wang C, et al.

**摘要**:通过X射线晶体学解析HK3重组蛋白的三维结构,发现其与HK1/HK2不同的底物结合域,为设计HK3特异性抑制剂提供了结构基础。

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如需具体文献,建议在PubMed、Web of Science等平台检索关键词“HK3 recombinant protein”或“Hexokinase 3 expression”。

背景信息

**Background of HK3 Recombinant Protein**

HK3. or hexokinase 3. is a member of the hexokinase family, a group of enzymes critical to glycolysis by catalyzing the phosphorylation of glucose to glucose-6-phosphate. Unlike its isoforms (HK1. HK2. and HK4), HK3 exhibits distinct tissue expression patterns, primarily found in leukocytes, lung, spleen, and placenta. It plays roles in energy metabolism, cellular proliferation, and apoptosis regulation. Dysregulation of HK3 has been linked to metabolic disorders and cancers, particularly due to its potential involvement in modulating immune responses and tumor microenvironments.

Recombinant HK3 protein is engineered via molecular cloning, often expressed in systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. Purification techniques, such as affinity chromatography with His-tags or GST tags, yield high-purity proteins for research. Its recombinant form retains enzymatic activity, enabling functional studies on substrate specificity, kinetic properties, and interactions with inhibitors or signaling molecules.

In biomedical research, HK3 recombinant protein serves as a tool to investigate metabolic reprogramming in cancer cells, immune cell activation, and inflammatory diseases. It also aids in drug discovery, screening for compounds targeting hexokinases to disrupt pathological glycolysis in tumors. Structural studies using recombinant HK3 contribute to understanding isoform-specific mechanisms, guiding the development of selective therapeutics.

Overall, HK3 recombinant protein bridges gaps in studying metabolic pathways and disease mechanisms, offering insights into therapeutic strategies for cancer and immune-related conditions. Its applications span basic research, diagnostics, and targeted therapy development.

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