Mouse Monoclonal ATG5 Antibody

The ATG5 antibody is a crucial tool for studying autophagy, a conserved cellular degradation process. ATG5 (Autophagy-Related 5) is a 33 kDa protein essential for autophagosome formation, acting as a key component of the ubiquitin-like conjugation system. It covalently binds to ATG12. forming the ATG12-ATG5 complex that facilitates phagophore membrane elongation during autophagy. Researchers use ATG5 antibodies to detect endogenous ATG5 protein levels, monitor autophagy activation/inhibition, and study its subcellular localization via techniques like Western blotting, immunofluorescence, and immunohistochemistry. These antibodies help investigate autophagy's role in physiological processes (cell differentiation, nutrient recycling) and pathological conditions, including cancer, neurodegenerative diseases (Alzheimer's, Parkinson's), and immune disorders. ATG5 knockout studies using specific antibodies have revealed its dual roles in autophagy-dependent cell survival and apoptosis regulation. Commercial ATG5 antibodies are typically developed in rabbit or mouse hosts, targeting specific epitopes (e.g., C-terminal regions). Validation includes testing in ATG5-deficient cell lines to confirm specificity. Proper antibody selection is critical as ATG5 exists in both free and conjugated (ATG12-bound) forms. Its detection aids in exploring therapeutic strategies targeting autophagy pathways.