| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TDT |
| Entrez GeneID | 1791 |
| clone | 4B10C3 |
| WB Predicted band size | 58.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DNTT (AA: 52-192) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于DNTT(末端脱氧核苷酸转移酶)抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Terminal deoxynucleotidyl transferase (TdT) as a marker for acute leukemia diagnosis: A comprehensive immunohistochemical study*
**作者**：Smith J, Doe R, Lee A.
**摘要**：本研究评估了DNTT(TdT)抗体在急性淋巴细胞白血病(ALL)诊断中的应用。通过免疫组化分析200例患者样本，证实TdT抗体在区分ALL与其他血液恶性肿瘤中具有高敏感性和特异性，尤其在未成熟淋巴细胞亚群中表达显著。
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2. **文献名称**：*Developmental regulation of TdT expression during lymphoid progenitor cell differentiation*
**作者**：Brown K, García-Sánchez P, Müller S.
**摘要**：该研究探讨了DNTT在淋巴细胞早期发育中的动态表达。利用流式细胞术和DNTT抗体标记，发现TdT在祖B细胞和T细胞前体阶段特异性表达，提示其在V(D)J重组中的关键作用，为免疫系统发育机制提供了新见解。
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3. **文献名称**：*Comparative analysis of commercial anti-TdT antibodies for flow cytometry: Sensitivity and clinical utility*
**作者**：Chen L, Wang H, O’Connor M.
**摘要**：比较了5种市售DNTT抗体的检测效能，发现克隆号HT-1和HT-3在流式细胞术中灵敏度最高，尤其适用于微量残留病(MRD)监测。研究为临床选择高特异性抗体提供了实验依据。
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以上文献涵盖了DNTT抗体在疾病诊断、基础研究及技术优化中的典型应用，均为示例性参考。如需实际引用，建议通过PubMed或学术数据库检索具体论文。
The DNA nucleotidylexotransferase (DNTT), also known as terminal deoxynucleotidyl transferase (TdT), is a specialized DNA polymerase that catalyzes the template-independent addition of nucleotides during V(D)J recombination, a critical process for generating diverse antigen receptors in developing B and T lymphocytes. Expressed predominantly in immature lymphoid precursors, DNTT plays a vital role in adaptive immunity by enhancing antibody and T-cell receptor diversity.
DNTT antibodies are immunodetection tools targeting this enzyme, widely utilized in research and diagnostics. In clinical pathology, DNTT antibodies serve as key markers for identifying precursor lymphoid neoplasms, such as acute lymphoblastic leukemias (ALL) and lymphoblastic lymphomas, as DNTT is aberrantly expressed in malignant lymphoid blasts but absent in mature lymphocytes. Its presence helps differentiate between immature and mature lymphoid malignancies, guiding treatment strategies.
In research, DNTT antibodies are employed to study lymphocyte development, recombination mechanisms, and immune repertoire formation. They are also used in flow cytometry, immunohistochemistry, and Western blotting to characterize hematopoietic progenitors in both normal and diseased states. Additionally, DNTT expression is explored in regenerative medicine for evaluating engineered lymphoid tissues. Despite its specificity, DNTT detection requires careful interpretation, as low-level expression may occur in non-lymphoid tissues under certain conditions.
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