| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TP; PMFA; VEGP |
| Entrez GeneID | 3933 |
| clone | 10B10 |
| WB Predicted band size | 19.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of LCN1 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
+ +
以下是3篇关于PLD2抗体的相关文献概览：
1. **文献名称**：*"Phospholipase D2-specific antibody inhibits endothelin-1-induced cardiomyocyte hypertrophy"*
**作者**：H. Zhang et al.
**摘要**：研究使用PLD2特异性抗体验证其在心肌细胞肥大信号通路中的作用，发现抗体阻断PLD2活性可抑制内皮素-1诱导的细胞肥大，提示PLD2是潜在治疗靶点。
2. **文献名称**：*"Characterization of a novel monoclonal antibody against phospholipase D2 for cancer cell migration studies"*
**作者**：K. Yamamoto & M. Kanaho
**摘要**：报道了一种新型PLD2单克隆抗体的开发，通过免疫印迹和免疫荧光验证其特异性，证明该抗体能抑制乳腺癌细胞迁移，为肿瘤转移机制研究提供工具。
3. **文献名称**：*"PLD2 regulates EGFR endocytosis through antibody-mediated functional inhibition in keratinocytes"*
**作者**：S. Gomez-Cambronero et al.
**摘要**：利用PLD2抗体阻断实验发现，PLD2通过调控表皮生长因子受体(EGFR)内吞影响角质细胞信号传导，揭示PLD2在膜运输中的关键作用。
注：以上文献为示例性内容，实际引用需以具体论文数据库(如PubMed)检索结果为准。
Phospholipase D2 (PLD2) is a member of the phospholipase D family, enzymes that catalyze the hydrolysis of phosphatidylcholine to generate phosphatidic acid (PA), a lipid second messenger involved in diverse cellular processes. PLD2 regulates key signaling pathways, including vesicle trafficking, cytoskeletal reorganization, cell proliferation, and survival. Its activity is modulated by interactions with receptors, small GTPases, and other signaling molecules. Dysregulation of PLD2 has been implicated in cancer progression, immune disorders, and neurodegenerative diseases, making it a potential therapeutic target.
PLD2 antibodies are essential tools for studying the expression, localization, and function of PLD2 in biological systems. These antibodies enable detection of endogenous or overexpressed PLD2 in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specificity is critical, as PLD2 shares structural homology with its isoform PLD1. High-quality antibodies distinguish between these isoforms, ensuring accurate research outcomes. Monoclonal and polyclonal PLD2 antibodies are widely used to explore PLD2’s role in cellular mechanisms, such as receptor tyrosine kinase signaling, membrane dynamics, and autophagy. They also aid in evaluating PLD2 inhibition strategies in disease models. Validating antibody specificity via knockout controls or peptide blocking is recommended to avoid off-target effects. Overall, PLD2 antibodies are indispensable for advancing our understanding of PLD2's physiological and pathological contributions.
×
