| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PMSL2; HNPCC4; PMS2CL |
| Entrez GeneID | 5395 |
| clone | 1E9D11 |
| WB Predicted band size | 95.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PMS2 (AA: 748-851) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是3篇关于PMS2抗体的代表性文献(信息基于领域内常见研究总结，非真实文献)：
---
1. **文献名称**：*PMS2 Immunohistochemistry in Colorectal Cancer: Current Practices and Impact on Lynch Syndrome Identification*
**作者**：Smith J, et al.
**摘要**：探讨PMS2抗体在结直肠癌组织中的免疫组化应用，分析其作为Lynch综合征筛查工具的敏感性和特异性，强调需联合其他错配修复蛋白(MLH1. MSH2. MSH6)检测以提高准确性。
---
2. **文献名称**：*Comparative Analysis of PMS2 Antibody Clones EP51 and EPR3947 in Diagnostic Pathology*
**作者**：Brown K, et al.
**摘要**：比较两种常用PMS2抗体克隆(EP51 vs. EPR3947)的性能，发现EPR3947在福尔马林固定组织中表现出更高的核染色特异性和稳定性，建议标准化使用以减少假阴性结果。
---
3. **文献名称**：*The Role of PMS2 Germline Mutations and Protein Expression in Endometrial Cancer*
**作者**：Lee H, et al.
**摘要**：研究PMS2抗体在子宫内膜癌中的表达缺失与胚系突变的关系，指出PMS2缺失常与MLH1启动子甲基化相关，需结合分子检测区分散发性与遗传性肿瘤。
---
*注：以上文献为示例性质，实际引用时请通过PubMed或学术数据库检索真实研究。*
The PMS2 antibody is a crucial tool in molecular pathology for assessing DNA mismatch repair (MMR) function. PMS2 encodes a protein component of the MutLα heterodimer, which partners with MLH1 to correct DNA replication errors. Germline mutations in PMS2 are linked to Lynch syndrome, a hereditary cancer predisposition syndrome associated with colorectal, endometrial, and other cancers. Immunohistochemistry (IHC) using PMS2 antibodies helps identify loss of protein expression in tumor tissues, indicating MMR deficiency (dMMR). This deficiency serves as a biomarker for microsatellite instability (MSI), guiding Lynch syndrome diagnosis, prognosis, and therapeutic decisions (e.g., immunotherapy response).
PMS2-specific antibodies are particularly valuable because its expression relies on MLH1 binding. In IHC, concurrent loss of PMS2 and MLH1 often suggests sporadic MLH1 promoter hypermethylation, whereas isolated PMS2 loss may indicate germline mutations. However, antibody specificity challenges arise due to homologous pseudogenes and protein cross-reactivity. Recent advancements in monoclonal antibody design have improved detection accuracy. Clinically, PMS2 IHC is part of a standard MMR panel (with MLH1. MSH2. MSH6) for colorectal and endometrial cancer screening. Its role extends to research on carcinogenesis, MMR mechanisms, and targeted therapies. Proper interpretation requires correlation with genetic testing and clinical history.
×
