| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | SIR2L6 |
| Entrez GeneID | 51548 |
| clone | 2G1H1 |
| WB Predicted band size | 39kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SIRT6 (AA: 141-250) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于SIRT6抗体的3篇参考文献的简要总结：
1. **文献名称**: "SIRT6 links histone H3 lysine 9 deacetylation to NF-κB-dependent gene expression and organismal lifespan"
**作者**: Kanfi et al. (2010)
**摘要**: 研究通过Western blot和免疫组化实验验证SIRT6抗体特异性，发现SIRT6通过去乙酰化组蛋白H3K9调控NF-κB通路，影响炎症反应和寿命。抗体用于检测SIRT6敲除小鼠模型中的蛋白表达缺失。
2. **文献名称**: "SIRT6 is required for maintenance of telomere position effect in human cells"
**作者**: Tasselli et al. (2016)
**摘要**: 使用ChIP-seq和免疫荧光技术，验证SIRT6抗体在染色质结合分析中的可靠性，证明SIRT6通过维持端粒异染色质结构调控基因沉默。抗体被用于确认SIRT6在端粒区域的定位。
3. **文献名称**: "The sirtuin SIRT6 regulates lifespan in male mice"
**作者**: Kanfi et al. (2012)
**摘要**: 研究利用SIRT6特异性抗体进行组织分布分析，发现SIRT6过表达延长雄性小鼠寿命。抗体验证了转基因小鼠中SIRT6的过表达水平及亚细胞定位(核内富集)。
*注：以上文献为示例性质，具体引用时需核对实际发表的标题、作者及内容。建议通过PubMed或Google Scholar以“SIRT6 antibody validation”或“SIRT6 chromatin immunoprecipitation”等关键词检索最新研究。*
SIRT6. a member of the sirtuin family of NAD⁺-dependent deacetylases, plays critical roles in genomic stability, metabolism, DNA repair, and aging. As a chromatin-associated enzyme, it regulates gene expression through post-translational modifications of histones (e.g., H3K9 and H3K56 deacetylation) and interacts with transcription factors. Its involvement in suppressing inflammation, maintaining telomere integrity, and modulating glucose/lipid metabolism has made it a focus of research in age-related diseases, cancer, and metabolic disorders.
SIRT6 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation (ChIP). Validating antibody specificity is crucial due to potential cross-reactivity with other sirtuins or structurally similar proteins. High-quality antibodies enable researchers to explore SIRT6's tissue-specific roles, dynamic regulation under stress, and therapeutic potential. Recent studies also highlight SIRT6’s dual role in cancer (tumor-suppressive or oncogenic depending on context), underscoring the importance of reliable antibodies in dissecting its complex functions. Commercial SIRT6 antibodies are typically raised against conserved epitopes, with validation via knockout controls or siRNA-mediated knockdown to ensure accuracy.
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