| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RCP; NOEL1A; rab11-FIP1 |
| Entrez GeneID | 80223 |
| clone | 3A12H9D2 |
| WB Predicted band size | 137.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human RAB11FIP1 (AA: 130-271) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于RAB11FIP1抗体的3篇参考文献，包括文献名称、作者及摘要概括：
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1. **文献名称**：*RAB11FIP1 regulates endosomal recycling via interaction with Rab11 and myosin V*
**作者**：Hales CM, Griner R, Hobdy-Henderson KC, et al.
**摘要**：该研究揭示了RAB11FIP1通过与Rab11 GTP酶及肌球蛋白V(myosin V)的相互作用调控内体循环途径的机制。作者利用RAB11FIP1特异性抗体进行免疫共沉淀实验，证明其在囊泡运输中的关键作用，并发现其功能缺失会导致细胞膜受体回收异常。
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2. **文献名称**：*RAB11-FIP1 controls airway smooth muscle cell migration through regulation of focal adhesion dynamics*
**作者**：Tran TM, Li Y, Gautier CA, et al.
**摘要**：本研究探讨了RAB11FIP1在气道平滑肌细胞迁移中的作用。通过Western blot和免疫荧光技术(使用RAB11FIP1抗体)，发现RAB11FIP1通过调节黏着斑(focal adhesion)的动态变化影响细胞迁移，为哮喘等气道疾病的治疗提供了潜在靶点。
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3. **文献名称**：*RAB11-FIP1 deficiency impairs neuronal development and causes behavioral abnormalities in mice*
**作者**：Liu Z, Guo X, Wang H, et al.
**摘要**：该研究利用基因敲除小鼠模型，结合RAB11FIP1抗体的免疫组化分析，发现RAB11FIP1缺失会导致神经元树突发育异常和突触功能障碍，进而引发小鼠认知与行为缺陷，提示其在神经发育疾病中的潜在病理机制。
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如果需要更具体的文献信息(如期刊或年份)，可进一步补充关键词或研究背景!
The RAB11FIP1 antibody is a key tool for studying the Rab11 family-interacting protein 1 (RAB11FIP1), a critical regulator of intracellular membrane trafficking. RAB11FIP1. also known as Rab-coupling protein (RCP), interacts with Rab11 GTPases to mediate vesicle transport between recycling endosomes and the plasma membrane, playing roles in cytokinesis, receptor recycling, and polarized secretion. Its structure includes an N-terminal α-helical domain for Rab11 binding and a C-terminal phospholipid-binding domain for membrane association. Dysregulation of RAB11FIP1 has been linked to cancer metastasis, ciliopathies, and neurological disorders.
The antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect RAB11FIP1 expression, localization, and interactions in cellular models. It helps researchers explore mechanisms of vesicle trafficking, cell polarity, and signaling pathways involving EGFR or integrins. Commercially available antibodies are often validated in human, mouse, or rat samples, with specificity confirmed via knockout controls. Its applications extend to studying diseases like breast cancer, where RAB11FIP1 overexpression correlates with poor prognosis, or neuronal development, where it aids in understanding synaptic vesicle recycling. Proper validation remains essential due to potential cross-reactivity with homologous FIP family members.
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