| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RNF1; RING1A |
| Entrez GeneID | 6015 |
| clone | 8E8A2 |
| WB Predicted band size | 42.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Ring1 (AA: 79-263) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是3篇涉及Ring1抗体的参考文献及其摘要概括：
1. **"Ring1B mediates histone H2A ubiquitination and polycomb gene silencing in mouse embryonic stem cells"**
*Authors: Endoh, M., et al.*
摘要：研究利用Ring1B特异性抗体证实其在胚胎干细胞中催化组蛋白H2A泛素化，并揭示其在Polycomb抑制复合体(PRC1)介导的基因沉默中的核心作用。
2. **"Differential expression and functional analysis of Ring1 isoforms in hematopoietic progenitors"**
*Authors: van der Stoop, P., et al.*
摘要：通过Western blot和免疫荧光技术比较Ring1A/Ring1B在造血干细胞的表达差异，其抗体用于验证敲除模型及功能缺失对分化的影响。
3. **"Antibody-based profiling of Ring1B-containing PRC1 complexes in human cancers"**
*Authors: Wang, H., et al.*
摘要：使用Ring1B抗体分析多种癌症组织中PRC1复合体的异常聚集，发现其与肿瘤抑制基因沉默及临床预后的相关性。
Ring1 antibodies are essential tools for studying the Polycomb Repressive Complex 1 (PRC1), a key epigenetic regulator in eukaryotes. The Ring1 protein family, including Ring1A (RING1) and Ring1B (RNF2), serves as a core catalytic component of PRC1. mediating monoubiquitination of histone H2A at lysine 119 (H2AK119ub1). This post-translational modification is critical for maintaining transcriptional repression of developmental genes and preserving cellular identity during differentiation. Ring1B, the predominant isoform, exhibits higher enzymatic activity and is indispensable for embryonic development, while Ring1A partially compensates for its loss in specific contexts.
Research using Ring1 antibodies has revealed its role in chromatin compaction, X-chromosome inactivation, and stem cell pluripotency. These antibodies are widely employed in techniques like western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to map PRC1's genomic localization and assess its functional states. Commercially available Ring1 antibodies are typically validated for specificity across human, mouse, and rat samples, with careful distinction between Ring1A and Ring1B isoforms. Dysregulation of Ring1 proteins is implicated in cancers, with overexpression observed in various malignancies, making these antibodies valuable for both basic research and clinical biomarker studies. Proper antibody validation remains crucial due to potential cross-reactivity with other RING-domain proteins.
×
