Recombinant Human CGA protein

中文名： 细胞周期蛋白A1(CGA)重组蛋白
别名： CGA；Cyclin-A1

货号: PA2000-4249

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	CGA
Uniprot No	P10645
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	19-457aa
氨基酸序列	LPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG
预测分子量	50.9 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于CGA(此处假设为绒毛膜促性腺激素α亚基)重组蛋白的模拟参考文献示例，供参考：

---

1. **标题**：High-yield Expression and Purification of Recombinant Human Chorionic Gonadotropin Alpha Subunit (CGA) in *E. coli*

**作者**：Zhang, L., Wang, Y., & Chen, H.

**摘要**：研究报道了通过大肠杆菌表达系统高效生产重组人CGA蛋白的优化方法，采用His标签纯化技术，获得了高纯度蛋白，并验证了其生物活性。

2. **标题**：Structural and Functional Characterization of Recombinant CGA for Cancer Immunotherapy

**作者**：Smith, J.R., et al.

**摘要**：通过X射线晶体学解析了重组CGA的三维结构，并证明其作为肿瘤疫苗佐剂的潜力，可增强抗原呈递细胞的免疫应答。

3. **标题**：Development of a Novel Glycosylated CGA Recombinant Protein for Reproductive Therapeutics

**作者**：Kim, S., & Gupta, R.

**摘要**：利用哺乳动物细胞表达系统(CHO细胞)成功制备了糖基化修饰的重组CGA蛋白，证实其在调节孕激素分泌中的临床应用价值。

4. **标题**：Comparative Analysis of CGA Expression Systems: Yeast vs. Mammalian Cell Platforms

**作者**：Martínez, F., et al.

**摘要**：对比酵母和哺乳动物细胞表达重组CGA的效率及产物差异，发现哺乳动物系统更适于保留蛋白天然构象和功能。

---

**注意**：以上文献为模拟示例，实际研究请通过PubMed、Google Scholar等平台检索关键词(如 "recombinant chorionic gonadotropin alpha" 或 "CGA protein expression")获取真实文献。

背景信息

**Background of CGA Recombinant Protein**

CGA (Chorionic Gonadotropin Alpha) recombinant protein is a biologically engineered subunit of human chorionic gonadotropin (hCG), a glycoprotein hormone critical in early pregnancy and reproductive functions. Naturally produced by placental syncytiotrophoblasts, hCG comprises two subunits: α (CGA) and β (CGB). The α-subunit is shared with other hormones like luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroid-stimulating hormone (TSH), highlighting its conserved structural role in hormone-receptor interactions.

The development of recombinant CGA leverages genetic engineering techniques, where the CGA gene is inserted into host systems (e.g., *E. coli*, yeast, or mammalian cells*) for scalable and consistent production. This approach overcomes limitations of traditional extraction methods, such as low yield and batch variability, while ensuring high purity and reduced immunogenicity.

CGA recombinant protein has broad applications. In research, it serves as a tool to study hormone signaling, pregnancy-related disorders, and tumor biology, as hCG is aberrantly expressed in certain cancers. Clinically, it is used in fertility treatments to trigger ovulation and support early pregnancy. Additionally, it aids in developing diagnostic assays, including pregnancy tests and cancer biomarker detection.

Recent advancements focus on optimizing glycosylation patterns (via mammalian expression systems) to enhance bioactivity and stability, as well as exploring its role in immunotherapies and vaccine design. CGA recombinant protein thus represents a cornerstone in both biomedical research and therapeutic innovation, bridging molecular biology with clinical needs.