| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5 |
| Entrez GeneID | 4090 |
| clone | 3H9 |
| WB Predicted band size | 52kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human SMAD5 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SMAD5抗体的3篇代表性文献的简要总结(注：内容为模拟示例，非真实文献)：
1. **文献名称**：*SMAD5 regulates embryonic stem cell differentiation through BMP signaling*
**作者**：Li et al.
**摘要**：研究利用SMAD5特异性抗体，通过Western blot和免疫荧光技术，证明SMAD5在BMP信号通路介导的胚胎干细胞向中胚层分化中起关键作用，并揭示其蛋白表达动态变化。
2. **文献名称**：*SMAD5 antibody validation in TGF-β-mediated cancer metastasis*
**作者**：Zhang et al.
**摘要**：该研究系统验证了SMAD5抗体的特异性(包括敲除细胞系对照)，发现SMAD5在TGF-β诱导的乳腺癌细胞上皮-间质转化(EMT)中高表达，并通过染色质免疫沉淀(ChIP)证明其与靶基因启动子结合。
3. **文献名称**：*Dual role of SMAD5 in bone formation: Insights from immunostaining*
**作者**：Garcia-Ruiz et al.
**摘要**：通过免疫组化及抗体阻断实验，揭示SMAD5在成骨细胞分化中的双重调控机制——既促进早期分化又抑制过度矿化，为骨代谢疾病治疗提供新靶点。
4. **文献名称**：*CRISPR screening identifies SMAD5 as a novel modulator of cardiac hypertrophy*
**作者**：Wang et al.
**摘要**：结合CRISPR筛选和SMAD5抗体流式细胞术分析，发现SMAD5通过抑制mTORC1通路缓解病理性心肌肥厚，为抗体在心血管疾病机制研究中的应用提供范例。
(提示：实际文献需通过PubMed/Google Scholar以“SMAD5 antibody”等关键词检索，建议结合抗体克隆号或应用场景进一步筛选。)
SMAD5 antibody is a crucial tool in studying the TGF-β/BMP signaling pathway, which regulates diverse cellular processes, including proliferation, differentiation, apoptosis, and embryonic development. SMAD5. a member of the receptor-regulated SMAD (R-SMAD) family, is primarily activated by bone morphogenetic proteins (BMPs) through phosphorylation of its C-terminal SSXS motif. Upon activation, SMAD5 forms complexes with SMAD4 and translocates to the nucleus to modulate target gene expression.
Antibodies targeting SMAD5 are widely used in research to detect its expression, phosphorylation status, and subcellular localization. These antibodies are essential for techniques like Western blotting, immunoprecipitation, and immunohistochemistry, enabling the investigation of SMAD5’s role in developmental biology, cancer (e.g., metastasis, resistance), and fibrotic diseases. Specificity is critical, as SMAD5 shares structural homology with other R-SMADs (e.g., SMAD1/8), requiring antibodies to distinguish unique epitopes. Validation via knockout controls or siRNA knockdown ensures reliability.
Research applications often focus on SMAD5’s dual roles: as a tumor suppressor in certain contexts or a promoter of pathological fibrosis. Dysregulation of SMAD5 has been linked to BMP-related disorders, such as vascular anomalies and skeletal defects. The antibody’s utility extends to drug discovery, where SMAD5 activity serves as a biomarker for therapeutic targeting in BMP pathway-associated diseases.
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